Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,601,831	(A)7→6	coding (342/1536 nt)	lsrA →	Autoinducer‑2 ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,601,825	0	A	.	100.0%	81.5 / NA	20	coding (336/1536 nt)	lsrA	Autoinducer‑2 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/9);  total (11/9)

GGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGG  >  NC_000913/1601748‑1601901                                                                              |                                                                             ggTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCaa                                                                             <  2:266156/79‑1 (MQ=255)       tATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCaaaaaacagc                                                                     >  2:266181/1‑77 (MQ=255)        aTCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGct                                                                   >  2:266179/1‑81 (MQ=255)              ttCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCAt                                                             <  1:266157/81‑1 (MQ=255)                ccccAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATg                                                            >  2:266190/1‑80 (MQ=255)                   cAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAg                                                         >  1:266177/1‑80 (MQ=255)                       aaCCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAAt                                                    >  2:266183/1‑81 (MQ=255)                                   tCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGc                                         <  2:266158/80‑1 (MQ=255)                                     ccAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTgg                                      >  2:266188/1‑81 (MQ=255)                                      cAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTggc                                     <  2:266159/81‑1 (MQ=255)                                         gCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTggcggc                                  <  2:266160/81‑1 (MQ=255)                                           cTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGgcgc                                >  1:266184/1‑81 (MQ=255)                                              tCGATAAAAGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTgg                             <  2:266161/81‑1 (MQ=255)                                                     aaGAAAACATCCTGTTTGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGc                        >  1:266187/1‑79 (MQ=255)                                                                cTGTTTGGGCTGGC‑AAAAAACAACTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTg            <  1:266162/80‑1 (MQ=255)                                                                   tttGGGCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCa          >  2:266191/1‑79 (MQ=255)                                                                      gggCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGt      <  1:266163/80‑1 (MQ=255)                                                                      gggCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTc     >  2:266200/1‑81 (MQ=255)                                                                       ggCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCt    <  2:266164/81‑1 (MQ=255)                                                                        gCTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTg   <  1:266165/81‑1 (MQ=255)                                                                         cTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTgg  >  1:266196/1‑81 (MQ=255)                                                                         cTGGC‑AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTgg  >  2:266189/1‑81 (MQ=255)                                                                              |                                                                             GGTATTTATCTCGTTCCCCAGGAACCGCTGCTTTTCCCAAGCCTGTCGATAAAAGAAAACATCCTGTTTGGGCTGGCAAAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGG  >  NC_000913/1601748‑1601901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑