Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,674,767	Δ1 bp	coding (855/1035 nt)	tqsA →	autoinducer 2 exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,674,764	0	G	.	100.0%	59.2 / NA	15	coding (852/1035 nt)	tqsA	autoinducer 2 exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (6/9);  total (6/9)

GAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTG  >  NC_000913/1674690‑1674827                                                                           |                                                                gAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATgggcgtg                                                           >  1:274285/1‑77 (MQ=255)       ttGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTgg                                                    <  1:274265/81‑1 (MQ=255)             gtgctggCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCtt                                               <  1:274266/80‑1 (MQ=255)                     gggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATtg                                      >  1:274289/1‑81 (MQ=255)                     gggATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATtg                                      >  1:274293/1‑81 (MQ=255)                                  aTTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGt                         >  1:274296/1‑81 (MQ=255)                                     aaTCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCg                       <  1:274267/80‑1 (MQ=255)                                           gTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAttt                >  1:274294/1‑81 (MQ=255)                                           gTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAtt                 <  1:274269/80‑1 (MQ=255)                                           gTCTTCGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAt                  <  1:274268/79‑1 (MQ=255)                                                cGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGAtttttt             <  2:274270/79‑1 (MQ=255)                                                cGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTgg           >  1:274292/1‑81 (MQ=255)                                                cGGCAATATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTg            <  1:274271/80‑1 (MQ=255)                                                    aaTATTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGAt       >  2:274297/1‑81 (MQ=255)                                                     atatTCTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATg      <  2:274272/81‑1 (MQ=255)                                                          cTGGAGCCGCGTATCAT‑GGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGgttg  <  2:274273/80‑1 (MQ=255)                                                                           |                                                                GAAGCGTTGCTGGTGCTGGCGGGATATCTGCTGATTAATCTGGTCTTCGGCAATATTCTGGAGCCGCGTATCATGGGGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTG  >  NC_000913/1674690‑1674827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑