Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,712,286	(A)5→4	intergenic (+128/‑483)	nth → / → dtpA	endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,712,282	0	A	.	100.0%	68.1 / NA	17	intergenic (+124/‑487)	nth/dtpA	endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (8/9);  total (8/9)

TTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTG  >  NC_000913/1712210‑1712351                                                                         |                                                                      ttAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCaaaatgc                                                                 <  1:278793/79‑4 (MQ=255)    aaaCATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGt                                                            <  2:278794/80‑1 (MQ=255)     aaCATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGtt                                                           >  2:278809/1‑80 (MQ=255)             tttAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGtttt                                                  >  2:278820/1‑81 (MQ=255)              ttAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGtttt                                                  <  1:278795/80‑1 (MQ=255)                   gTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAAt                                            <  2:278796/81‑1 (MQ=255)                   gTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAAt                                            <  2:278797/81‑1 (MQ=255)                             ccATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAAttt                                  >  1:278814/1‑81 (MQ=255)                             ccATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAAtt                                   <  1:278798/80‑1 (MQ=255)                               aTTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTc                                >  1:278821/1‑81 (MQ=255)                                  gCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTat                             >  1:278825/1‑81 (MQ=255)                                        cGTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTattcattc                       <  2:278799/81‑1 (MQ=255)                                         gTTATCATCAGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCg                      >  2:278819/1‑81 (MQ=255)                                               atcaGGTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATc                 >  1:278816/1‑80 (MQ=255)                                                   ggTAATCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGAt            <  2:278800/81‑1 (MQ=255)                                                       aTCCGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCAc         <  1:278801/80‑1 (MQ=255)                                                          cGTTTGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATg     >  2:278818/1‑81 (MQ=255)                                                              tGCGGATAACC‑AAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTg  <  2:278802/80‑1 (MQ=255)                                                                         |                                                                      TTAAAACATCTTTTTAACGTCAATGATGCCATTGCTTAGCGTTATCATCAGGTAATCCGTTTGCGGATAACCAAAAATGCAGGTTAATTGTTTTTTTAATAGCGAAATTTTCTATTCATTCGTGATCAAGATCACGATGCTG  >  NC_000913/1712210‑1712351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑