Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,524	Δ1 bp	coding (721/1437 nt)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,521	0	C	.	100.0%	95.5 / NA	23	coding (724/1437 nt)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/10);  total (13/10)

GTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGA  >  NC_000913/1790443‑1790597                                                                               |                                                                             gTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCAcc                                                                             >  2:288525/1‑80 (MQ=255) gTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCAAGCAGCGACATGTTGTCAGTATTCATCAccc                                                                            <  2:288506/81‑1 (MQ=255)          catcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCa                                                                    >  2:288538/1‑79 (MQ=255)               aGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGa                                                             >  1:288548/1‑81 (MQ=255)                     ccAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCt                                                       <  2:288507/81‑1 (MQ=255)                          cGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCAt                                                  >  2:288549/1‑81 (MQ=255)                           ggCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCAtt                                                 <  2:288508/81‑1 (MQ=255)                           ggCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCAtt                                                 >  1:288537/1‑81 (MQ=255)                                     cAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTaa                                       >  2:288539/1‑81 (MQ=255)                                      aaGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAac                                      >  2:288547/1‑81 (MQ=255)                                           tCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGAcgc                                 <  2:288509/81‑1 (MQ=255)                                            cAGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGAcgcg                                >  1:288543/1‑81 (MQ=255)                                             aGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGAcgcg                                <  1:288510/80‑1 (MQ=255)                                             aGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGAcgcg                                <  2:288511/80‑1 (MQ=255)                                             aGCCCCAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCgg                               >  1:288536/1‑81 (MQ=255)                                               ccccAGCAGCGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTa                             >  1:288535/1‑81 (MQ=255)                                                   agcagcGACATGTTGTCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTg                         >  2:288546/1‑81 (MQ=255)                                                          aCATGTTGTCGGTATTCATCA‑CCCATGAGCAGAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGacaac                  <  2:288512/81‑1 (MQ=255)                                                             tgttgtCGGTATTCAACA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATc               <  1:288513/81‑1 (MQ=255)                                                               ttgtCGGTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGc             <  1:288514/81‑1 (MQ=255)                                                                     gTATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCGATTAACGACGCGGTACGTGCGACAACATCGCTAAAc        >  1:288544/1‑80 (MQ=255)                                                                      tATTCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCa      <  2:288515/81‑1 (MQ=255)                                                                        ttCATCA‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCaga    <  1:288516/81‑1 (MQ=255)                                                                         tcatca‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCa      >  2:288545/1‑78 (MQ=255)                                                                          catca‑CCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAgaga  >  2:288541/1‑81 (MQ=255)                                                                               |                                                                             GTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGA  >  NC_000913/1790443‑1790597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑