Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,807,314	(C)5→4	intergenic (+15/‑86)	pfkB → / → ydiZ	6‑phosphofructokinase II/protein YdiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,310	0	C	.	100.0%	72.5 / NA	18	intergenic (+11/‑90)	pfkB/ydiZ	6‑phosphofructokinase II/protein YdiZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/6);  total (12/6)

AATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGA  >  NC_000913/1807234‑1807382                                                                             |                                                                         aaTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccc                                                                        >  1:290549/1‑79 (MQ=255)            acGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGa                                                          <  1:290531/81‑1 (MQ=255)              gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAat                                                        >  2:290563/1‑81 (MQ=255)                        ccATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAAc                                              <  1:290532/81‑1 (MQ=255)                             aCGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGt                                          <  2:290533/80‑1 (MQ=255)                             aCGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTc                                         >  1:290559/1‑81 (MQ=255)                               gATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCgg                                       >  1:290565/1‑81 (MQ=255)                                    gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcg                                  >  1:290550/1‑81 (MQ=255)                                    gCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcg                                  <  1:290534/81‑1 (MQ=255)                                     cAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcgc                                 >  1:290553/1‑81 (MQ=255)                                        aaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGtt                              >  2:290556/1‑81 (MQ=255)                                         aaaTTTACGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGttt                             <  1:290535/81‑1 (MQ=255)                                               aCGCTTACCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGa                       >  2:290555/1‑81 (MQ=255)                                                     aCCTTTCCCGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGc                 >  1:290560/1‑81 (MQ=255)                                                           cccGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaa             >  1:290568/1‑79 (MQ=255)                                                           cccGCTAACAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaa             >  2:290557/1‑79 (MQ=255)                                                                  aCAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtg     <  2:290536/80‑1 (MQ=255)                                                                   cAAAAACATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAtgt    >  2:290558/1‑80 (MQ=255)                                                                     aaaaCATT‑CCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGa  >  2:290564/1‑80 (MQ=255)                                                                             |                                                                         AATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGA  >  NC_000913/1807234‑1807382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑