Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,177	(T)6→5	coding (358/699 nt)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,172	0	T	.	100.0%	134.4 / NA	32	coding (363/699 nt)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (22/10);  total (22/10)

CGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTA  >  NC_000913/1915092‑1915248                                                                                 |                                                                             cggcgTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGt                                                                              >  2:304615/1‑81 (MQ=255)  ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtt                                                                             >  1:304600/1‑81 (MQ=255)   gcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttt                                                                            <  1:304563/81‑1 (MQ=255)     gtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttttt                                                                          >  1:304617/1‑81 (MQ=255)      tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttc                                                                         <  1:304564/81‑2 (MQ=255)        gcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttcgc                                                                       >  1:304618/1‑78 (MQ=255)            aGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGc                                                                  >  1:304623/1‑81 (MQ=255)            aGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGc                                                                  >  2:304606/1‑81 (MQ=255)             gTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGc                                                                  <  1:304565/80‑1 (MQ=255)             gTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCt                                                                 >  2:304624/1‑81 (MQ=255)               cGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTAGCTGt                                                               >  2:304614/1‑81 (MQ=255)                ggACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGtt                                                              <  1:304566/81‑1 (MQ=255)                ggACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGtt                                                              >  2:304596/1‑81 (MQ=255)                 gACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTc                                                             <  1:304567/81‑1 (MQ=255)                  aCGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCa                                                            <  2:304568/81‑1 (MQ=255)                     tGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCa                                                         >  2:304612/1‑81 (MQ=255)                       gCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACg                                                       >  2:304629/1‑81 (MQ=255)                           gCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCtgt                                                   >  1:304603/1‑81 (MQ=255)                             gTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGc                                                 >  1:304626/1‑81 (MQ=255)                              ttCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTTcc                                                <  1:304569/81‑1 (MQ=255)                                 gcggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                                              >  1:304610/1‑80 (MQ=255)                                   ggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                            >  2:304604/1‑80 (MQ=255)                                    gcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAaca                                          >  1:304631/1‑81 (MQ=255)                                      gcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcg                                        <  1:304570/81‑1 (MQ=255)                                       cggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgc                                       >  1:304602/1‑81 (MQ=255)                                        ggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgcg                                      >  1:304611/1‑81 (MQ=255)                                          tGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCt                                    >  1:304609/1‑81 (MQ=255)                                           gCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCtt                                   >  1:798152/1‑81 (MQ=255)                                           gCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCtt                                   >  2:304605/1‑81 (MQ=255)                                            cGTCTT‑TTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                                 <  2:304571/81‑1 (MQ=255)                                             gTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                                 <  1:304572/81‑1 (MQ=255)                                             gTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                                 >  1:304616/1‑81 (MQ=255)                                             gTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                                 >  1:304608/1‑81 (MQ=255)                                              tcttctTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCg                                >  1:304630/1‑81 (MQ=255)                                                  ctttctCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGctt                             <  1:304573/80‑1 (MQ=255)                                                          ccAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgct                    >  2:304622/1‑81 (MQ=255)                                                                    cGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGc          <  2:304574/81‑1 (MQ=255)                                                                            tCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTTGCTTCTTCAAGCTGCTTGCGAGCATGCTCTa  >  2:304633/1‑81 (MQ=255)                                                                                 |                                                                             CGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGCTCTA  >  NC_000913/1915092‑1915248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑