Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,946,197	(A)6→5	intergenic (‑17/‑54)	yobI ← / → yebB	uncharacterized protein YobI/putative papain‑like amidase YebB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,946,192	0	A	.	100.0%	41.3 / NA	11	intergenic (‑12/‑59)	yobI/yebB	uncharacterized protein YobI/putative papain‑like amidase YebB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (7/4);  total (7/4)

GCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCC  >  NC_000913/1946126‑1946267                                                                   |                                                                            gCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTG‑AAAAATAAAttttta                                                              >  1:308948/1‑80 (MQ=255)        ttttAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTG‑AAAAATAAA‑TTTTTATGCTAt                                                       <  1:308938/80‑1 (MQ=255)                 tACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTG‑AAAAATAAA‑TTTTTATGCTATTACGTATAtt                                             >  1:308955/1‑81 (MQ=255)                 tACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTG‑AAAAATAAA‑TTTTTATGCTATTACGTATAtt                                             >  2:308949/1‑81 (MQ=255)                                  aaTGCGTAATAAAAATATACATTGCGCCTCCTG‑AAAAATAAA‑TTTTTATGCTATTACGTATATTTGTATCTATTTCAATgg                            <  1:308939/81‑1 (MQ=255)                                      cGTAATAAAAATATACATTGCGCCTCCTG‑AAAAATAAA‑TTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAAt                         <  1:308940/80‑1 (MQ=255)                                       gTAATAAAAATATACATTGCGCCTCCTG‑AAAAATAAA‑TTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGa                       >  1:308951/1‑81 (MQ=255)                                             aaaaTATACATTGCGCCTCCTG‑AAAAAT‑AAGTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACg                  <  2:308941/80‑1 (MQ=255)                                                    aCATTGCGCCTCCTG‑AAAAATAAA‑TTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAatat           >  1:308957/1‑80 (MQ=255)                                                       ttGCGCCTCCTG‑AAAAATAAA‑TTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATattaat       >  2:308953/1‑81 (MQ=255)                                                            cctcctG‑AAAAATAAA‑TTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTAccc  >  2:308947/1‑81 (MQ=255)                                                                   |                                                                            GCAAATATTTTAATATTACATATTCAGTGAAGAAATGCGTAATAAAAATATACATTGCGCCTCCTGAAAAAATAAATTTTTTATGCTATTACGTATATTTGTATCTATTTCAATGGAATGACAACGTGAATATTAATTACCC  >  NC_000913/1946126‑1946267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑