Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,150,632	Δ1 bp	coding (354/1947 nt)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,150,629	0	A	.	100.0%	68.0 / NA	17	coding (357/1947 nt)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/7);  total (10/7)

TTTACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGAAAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTA  >  NC_000913/2150552‑2150701                                                                              |                                                                         tttACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGaaat                                                                       >  2:338349/1‑80 (MQ=255)    aCGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGaaatcc                                                                     >  1:338343/1‑77 (MQ=255)           ccACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGcac                                                             >  1:338362/1‑80 (MQ=255)              cgacgTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATg                                                         <  1:338328/81‑1 (MQ=255)               gacgTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAAt                                                          >  2:338357/1‑79 (MQ=255)                acgTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAg                                                       >  2:338356/1‑81 (MQ=255)                      ccGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAAc                                                 >  1:338361/1‑81 (MQ=255)                           cTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTgg                                            >  1:338354/1‑81 (MQ=255)                               tGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGcgac                                        <  1:338329/81‑1 (MQ=255)                                          aaaaGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCggg                             >  2:338365/1‑81 (MQ=255)                                            aaGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCt                           <  1:338330/81‑1 (MQ=255)                                              gATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCtat                         <  2:338331/81‑1 (MQ=255)                                                 gAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCta                          >  2:338351/1‑77 (MQ=255)                                                  aaGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGa                      <  2:338332/80‑1 (MQ=255)                                                     cAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCat                  <  1:338333/81‑1 (MQ=255)                                                                  aaCATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCt     >  1:338359/1‑81 (MQ=255)                                                                    cATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCttt   <  2:338334/81‑1 (MQ=255)                                                                     aTAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTa  >  1:338364/1‑81 (MQ=255)                                                                     aTAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTa  >  1:338355/1‑81 (MQ=255)                                                                              |                                                                         TTTACGTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGAAAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTA  >  NC_000913/2150552‑2150701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑