Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,183,325	Δ1 bp	coding (327/819 nt)	yegX ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,183,322	0	G	.	100.0%	93.6 / NA	23	coding (330/819 nt)	yegX	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (14/9);  total (14/9)

ATACCGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTT  >  NC_000913/2183243‑2183395                                                                                |                                                                          aTACCGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAgg                                                                          >  2:343419/1‑81 (MQ=255)     cGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAgggtcc                                                                      <  2:343400/81‑4 (MQ=255)      gACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAgggtcca                                                                     >  1:343428/1‑77 (MQ=255)         ggggAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTccac                                                                   >  1:343434/1‑79 (MQ=255)           ggAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGc                                                               >  1:343427/1‑81 (MQ=255)             agaAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCtt                                                             <  1:343401/81‑1 (MQ=255)              gaAATAGTGATACGCCCCACGCAGCAGGGCAGTGTCGCGGCTGAGTGGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCttt                                                            <  2:343402/81‑1 (MQ=255)               aaaTAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACCCGAAAAATA‑GGGTCCACCAGCtttt                                                           >  2:343424/1‑81 (MQ=255)                    atGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGcc                                                       <  1:343403/80‑1 (MQ=255)                        tACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCg                                                  >  2:343439/1‑81 (MQ=255)                            ccccACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGc                                              >  1:343438/1‑81 (MQ=255)                             cccACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGcc                                             <  2:343404/81‑1 (MQ=255)                               cACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCt                                            >  1:343436/1‑80 (MQ=255)                                       agGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAg                                   >  2:343445/1‑81 (MQ=255)                                        ggCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCAGTCGCCTTAATAAAAg                                   >  1:343425/1‑80 (MQ=255)                                         gCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGc                                  >  2:343433/1‑80 (MQ=255)                                          ccATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCaa                                <  2:343405/81‑1 (MQ=255)                                            aTTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAAc                              >  2:343430/1‑81 (MQ=255)                                                         aGTTGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATAc                 >  1:343440/1‑81 (MQ=255)                                                           ttGCCAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCa               <  2:343406/81‑1 (MQ=255)                                                              ccAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCAtt             <  1:343407/80‑1 (MQ=255)                                                               cAGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGt           >  2:343449/1‑81 (MQ=255)                                                                aGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTc          <  1:343408/81‑1 (MQ=255)                                                                aGTTACGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTc          <  1:343409/81‑1 (MQ=255)                                                                    aCGCGAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTcgcg       >  2:343443/1‑80 (MQ=255)                                                                        gAAAAATA‑GGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCAttt  >  2:343444/1‑81 (MQ=255)                                                                                |                                                                          ATACCGACGGGGAGAAATAATGATACGCCCCACGCAGCAGGCCATTTTCGCGGCTTAGTTGCCAGTTACGCGAAAAATAGGGGTCCACCAGCTTTTCGCCTTCCGTCGCCTTAATAAAAGCAAACTGTAAGCGGATACCATTGTCGCGCATTT  >  NC_000913/2183243‑2183395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑