Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,273,494	(A)6→5	coding (1131/1815 nt)	yejA →	putative oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,273,489	0	A	.	100.0%	104.0 / NA	25	coding (1126/1815 nt)	yejA	putative oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/12);  total (13/12)

GAACAGTTATTTTCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATGAAAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGCGATGGCTACGATC  >  NC_000913/2273410‑2273561                                                                                |                                                                         gAACAGTTATTTTCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATGa                                                                          >  1:357466/1‑80 (MQ=255)   aCAGTTATTTTCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATGaaaa                                                                       <  2:357431/81‑1 (MQ=255)    cAGTTATTTTCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATGaaa                                                                        >  1:357453/1‑79 (MQ=255)    cAGTTATTTTCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATGaaa                                                                        >  2:357458/1‑79 (MQ=255)     aGTTATTTTCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATGaaa                                                                        >  2:357451/1‑78 (MQ=255)       ttATTTTCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATGaaaaagat                                                                   <  2:357432/81‑4 (MQ=255)            ttCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTAc                                                              <  1:357433/80‑1 (MQ=255)             tCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTAcc                                                             <  2:357434/80‑1 (MQ=255)               aGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTc                                                          <  1:357435/81‑1 (MQ=255)                            cgcgGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTcac                                              <  2:357436/80‑1 (MQ=255)                             gcgGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTcacac                                            <  1:357437/81‑1 (MQ=255)                             gcgGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTcaca                                             >  1:357473/1‑80 (MQ=255)                                   aGAAATTACCCCGACGCCGCTGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATc                                       >  2:357467/1‑80 (MQ=255)                                   aGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCt                                      <  1:357438/81‑1 (MQ=255)                                       aTTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTAc                                    >  1:357465/1‑79 (MQ=255)                                        ttACCCCGACGCCGCGGAGCCGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAAGCTACCAg                                 >  1:357460/1‑81 (MQ=255)                                           ccccGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAgccg                              >  2:357478/1‑81 (MQ=255)                                             ccGACGCCGCGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAgccgcc                            >  1:357472/1‑81 (MQ=255)                                                  gccgcGGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTAt                       >  1:357462/1‑81 (MQ=255)                                                    cgcgGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATcc                     <  1:357439/81‑1 (MQ=255)                                                     gcgGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATcc                     >  1:357474/1‑80 (MQ=255)                                                      cgGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCaa                   <  2:357440/81‑1 (MQ=255)                                                      cgGAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCa                    >  1:357469/1‑80 (MQ=255)                                                       ggAGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCa                    >  1:357452/1‑79 (MQ=255)                                                         aGCTGGTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAgg                <  2:357441/81‑1 (MQ=255)                                                              gTGCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGCGATg           >  1:357484/1‑81 (MQ=255)                                                                gCTTCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGCGATGGc         >  2:357487/1‑81 (MQ=255)                                                                  ttCTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGCGATGGCTa       >  2:357475/1‑81 (MQ=255)                                                                    cTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGCGATGGCTAc      <  1:357443/80‑1 (MQ=255)                                                                    cTGGCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGCGATGGCTAc      <  1:357442/80‑1 (MQ=255)                                                                       gCACCAATG‑AAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGCGATGGCTACGATc  <  1:357444/81‑1 (MQ=255)                                                                                |                                                                         GAACAGTTATTTTCAGAATACCGAATACGCGGCCAGAAATTACCCCGACGCCGCGGAGCTGGTGCTTCTGGCACCAATGAAAAAAGATCTACCGTCAGAAGTCTTCACACAAATCTACCAGCCGCCGGTATCCAAAGGCGATGGCTACGATC  >  NC_000913/2273410‑2273561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑