Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,592,808	(T)5→4	coding (47/201 nt)	ypfN →	UPF0370 protein YpfN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,592,804	0	T	.	100.0%	98.7 / NA	24	coding (43/201 nt)	ypfN	UPF0370 protein YpfN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/14);  total (10/14)

GTCGCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAA  >  NC_000913/2592726‑2592874                                                                               |                                                                       gtcgCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCttt                                                                      <  2:412360/81‑1 (MQ=255) gtcgCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCttt                                                                      >  1:412413/1‑81 (MQ=255)    gCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCttttgg                                                                   <  1:412361/81‑3 (MQ=255)    gCCTGATGAGCGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCttttgg                                                                   >  2:412407/1‑79 (MQ=255)         atgaGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAg                                                               <  1:412362/79‑1 (MQ=255)         atgaGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGc                                                             >  1:412401/1‑81 (MQ=255)            aGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGt                                                           <  1:412363/80‑1 (MQ=255)               ggTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCctg                                                       >  1:412390/1‑81 (MQ=255)                      cAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGa                                                   <  1:412364/78‑1 (MQ=255)                           ggAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGAtt                                           <  2:412365/81‑1 (MQ=255)                                    cATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGAtctc                                  <  1:412366/81‑1 (MQ=255)                                    cATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGAtct                                   >  2:412412/1‑80 (MQ=255)                                        gACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAgc                              >  2:412405/1‑81 (MQ=255)                                        gACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAgc                              <  2:412368/81‑1 (MQ=255)                                        gACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAg                               <  2:412367/80‑1 (MQ=255)                                         aCTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAgcg                             >  1:412403/1‑81 (MQ=255)                                         aCTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAgcg                             <  1:412369/81‑1 (MQ=255)                                          ctggctggCTAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAgcgc                            <  2:412370/81‑1 (MQ=255)                                                 gctAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGAcc                     <  2:412371/81‑1 (MQ=255)                                                 gctAAATATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGAc                      <  2:412372/80‑1 (MQ=255)                                                    aaaTATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCAt                   >  1:412406/1‑80 (MQ=255)                                                     aaTATTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCAt                   <  1:412373/79‑1 (MQ=255)                                                        atTGGTGGATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGa               >  2:412402/1‑80 (MQ=255)                                                            gtggATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAAtt           <  1:412374/80‑1 (MQ=255)                                                               gATTCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTtctc       >  2:412422/1‑81 (MQ=255)                                                                 ttCTGGTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGc     >  2:412408/1‑81 (MQ=255)                                                                     ggTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCcaa  >  2:412428/1‑80 (MQ=255)                                                                      gTGATTGTC‑TTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCca   <  1:412375/78‑1 (MQ=255)                                                                               |                                                                       GTCGCCTGATGAGTGGTTCTGCAAGAGGAAATAAGCATGGACTGGCTGGCTAAATATTGGTGGATTCTGGTGATTGTCTTTTTGGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAA  >  NC_000913/2592726‑2592874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑