Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,223	(T)5→4	coding (406/2019 nt)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,219	0	T	.	100.0%	117.3 / NA	28	coding (402/2019 nt)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/12);  total (16/12)

CAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACG  >  NC_000913/2602141‑2602294                                                                               |                                                                            cAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCttt                                                                           <  2:414158/81‑1 (MQ=255)          ggcggcgATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATc                                                                 <  2:414159/81‑1 (MQ=255)            cggcgATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCCGGTGATGGACAACGC‑TTTTGGTTCATCGt                                                               >  2:414182/1‑81 (MQ=255)             ggcgATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTg                                                              >  1:414210/1‑81 (MQ=255)             ggcgATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTg                                                              <  2:414160/81‑1 (MQ=255)              gcgATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGc                                                             >  2:414207/1‑81 (MQ=255)                    ggCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTg                                                       <  1:414161/81‑1 (MQ=255)                        tcttTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAat                                                   >  1:414200/1‑81 (MQ=255)                        tcttTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAat                                                   >  2:414199/1‑81 (MQ=255)                           ttATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAatgat                                                >  2:414213/1‑81 (MQ=255)                            tATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAacgatg                                               >  1:414208/1‑81 (MQ=255)                                aaTATTTTCATCGCATCGATGGTTGCCCTGCAGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGc                                           <  2:414162/81‑1 (MQ=255)                                    ttttCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTc                                       >  2:414206/1‑81 (MQ=255)                                        catcgcatcgATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTcc                                   >  2:414217/1‑81 (MQ=255)                                         atcgcatcgATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTcc                                   <  2:414164/80‑1 (MQ=255)                                         atcgcatcgATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCt                                  <  1:414163/81‑1 (MQ=255)                                           cgcatcgATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTgg                                >  1:414209/1‑81 (MQ=255)                                            gcatcgATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGt                               >  1:414223/1‑81 (MQ=255)                                            gcatcgATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGt                               >  2:414215/1‑81 (MQ=255)                                               tcgATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTc                            >  1:414240/1‑81 (MQ=255)                                                cgATGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCt                           >  1:414218/1‑81 (MQ=255)                                                   tGGTTGCCCTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGt                        <  1:414165/81‑1 (MQ=255)                                                         cccTGCTGGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTg                   <  2:414166/80‑1 (MQ=255)                                                             gctgGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCcagg              >  2:414219/1‑81 (MQ=255)                                                              ctgGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCcag               <  2:414167/79‑1 (MQ=255)                                                              ctgGTGATGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCcag               <  1:414168/79‑1 (MQ=255)                                                                     tGGACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATaa      <  1:414169/81‑1 (MQ=255)                                                                       gACAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATaaa     <  2:414170/80‑1 (MQ=255)                                                                         cAACGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACg  >  2:414212/1‑81 (MQ=255)                                                                               |                                                                            CAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACG  >  NC_000913/2602141‑2602294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑