Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,542	Δ1 bp	coding (1632/2574 nt)	clpB ←	ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,539	0	C	.	100.0%	129.8 / NA	31	coding (1635/2574 nt)	clpB	ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/19);  total (12/19)

AATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTT  >  NC_000913/2732461‑2732615                                                                               |                                                                             aaTTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATccc                                                                            <  1:439427/81‑1 (MQ=255) aaTTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATccc                                                                            <  1:439426/81‑1 (MQ=255)      cgcgATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATcccgtcc                                                                        <  2:439429/80‑5 (MQ=255)      cgcgATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCa                                                                      <  2:439428/81‑1 (MQ=255)         gATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAAcg                                                                   <  2:439430/81‑1 (MQ=255)              tGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCa                                                              <  1:439432/81‑1 (MQ=255)               gCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAg                                                             >  2:439476/1‑81 (MQ=255)                cAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGc                                                            <  2:439433/81‑1 (MQ=255)                  gTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCAc                                                          <  1:439434/81‑1 (MQ=255)                    tCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACtt                                                        >  1:439491/1‑81 (MQ=255)                        gCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTc                                                       <  2:439436/78‑1 (MQ=255)                        gCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTc                                                       <  1:439435/78‑1 (MQ=255)                          tCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCaa                                                  >  2:439473/1‑81 (MQ=255)                            cATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAAtt                                                <  1:439437/81‑1 (MQ=255)                              tACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTc                                              <  1:439438/81‑1 (MQ=255)                                   agcagTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGt                                         <  1:439439/81‑1 (MQ=255)                                   agcagTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGt                                         >  1:439475/1‑81 (MQ=255)                                      agTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCgg                                      >  2:439481/1‑81 (MQ=255)                                                gctcgctTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTttatt                            <  2:439441/81‑1 (MQ=255)                                                gctcgctTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTttatt                            <  2:439440/81‑1 (MQ=255)                                                    gctTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTAc                          <  2:439442/79‑1 (MQ=255)                                                     ctTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGc                        >  1:439503/1‑80 (MQ=255)                                                     ctTTCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCa                       <  1:439443/81‑1 (MQ=255)                                                        tCCATCATGCGAGAAACCGGAAT‑CCCGTCTAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCag                      >  2:439488/1‑78 (MQ=39)                                                        tCCATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAAc                     <  1:439444/80‑1 (MQ=255)                                                         ccATCATGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCaa                      >  2:439490/1‑78 (MQ=255)                                                             catGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACg                >  2:439493/1‑80 (MQ=255)                                                              atGCGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCa              >  1:439486/1‑81 (MQ=255)                                                                 cGAGAAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATa            <  2:439445/80‑1 (MQ=255)                                                                  gagaAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGt          >  1:439499/1‑81 (MQ=255)                                                                    gaAACCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGttt        <  2:439446/81‑1 (MQ=255)                                                                       aCCGGAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGc     <  1:439447/81‑1 (MQ=255)                                                                          ggAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCtt  >  1:439482/1‑81 (MQ=255)                                                                          ggAAT‑CCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCtt  <  1:439448/81‑1 (MQ=255)                                                                               |                                                                             AATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGAAACCGGAATCCCCGTCCAACGCGCCAGCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTT  >  NC_000913/2732461‑2732615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑