Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,758,557	(A)5→4	coding (44/957 nt)	yfjH ←	CP4‑57 prophage; protein YfjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,758,553	0	A	.	100.0%	76.7 / NA	19	coding (48/957 nt)	yfjH	CP4‑57 prophage; protein YfjH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (12/7);  total (12/7)

GTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAAT  >  NC_000913/2758477‑2758629                                                                             |                                                                             gtTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTaaaat                                                                          <  1:444349/81‑2 (MQ=255) gtTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTaaaat                                                                          >  2:444370/1‑80 (MQ=255) gtTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTaaaa                                                                           <  1:444348/80‑1 (MQ=255)      cTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGT‑AAAATTGTAt                                                                    >  1:444368/1‑81 (MQ=255)        aCAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGT‑AAAATTGTAttt                                                                  >  1:444375/1‑81 (MQ=255)             tttGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTgg                                                              >  2:444372/1‑80 (MQ=255)                   cATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATgg                                                       >  1:444376/1‑81 (MQ=255)                       aaaaTCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGtt                                                    <  2:444350/80‑1 (MQ=255)                             aTTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGc                                              <  2:444351/80‑1 (MQ=255)                                   agaAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAg                                       <  1:444352/81‑1 (MQ=255)                                                 caccaTAAATCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAAtct                         >  2:444380/1‑81 (MQ=255)                                                     aTAAATCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATctctct                     >  1:444374/1‑81 (MQ=255)                                                     aTAAATCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATctctct                     >  2:444384/1‑81 (MQ=255)                                                      tAAATCAATGTATTAGAGGTTGT‑AAAATTGTGTTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTg                    <  2:444353/81‑1 (MQ=255)                                                       aaaTCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTg                    >  2:444378/1‑80 (MQ=255)                                                       aaaTCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTg                    >  2:444379/1‑80 (MQ=255)                                                        aaTCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGc                   <  2:444354/80‑1 (MQ=255)                                                         aTCAATGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCaa                 >  2:444383/1‑81 (MQ=255)                                                             aTGTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCa              >  1:444382/1‑80 (MQ=255)                                                               gTATTAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAg           >  1:444381/1‑81 (MQ=255)                                                                  ttAGAGGTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGa        <  2:444355/81‑1 (MQ=255)                                                                        gTTGT‑AAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAAt  <  1:444356/81‑1 (MQ=255)                                                                             |                                                                             GTTTTCTACAAATTTGTTCATAAAAATCATTCAGAGAAATCATAAAACCACCATAAATCAATGTATTAGAGGTTGTAAAAATTGTATTTTTGGTACATGGGTTTTTTGCCTTAAAGATACCCATTAATCTCTCTGCAACCAAAGTGAACCAAT  >  NC_000913/2758477‑2758629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑