Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,898,999	Δ1 bp	coding (420/1455 nt)	ygcU ←	putative FAD‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,898,996	0	C	.	100.0%	103.5 / NA	25	coding (423/1455 nt)	ygcU	putative FAD‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/16);  total (9/16)

GAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCA  >  NC_000913/2898918‑2899072                                                                               |                                                                             gagTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGccc                                                                            <  2:471426/81‑1 (MQ=255) gagTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGcc                                                                             <  2:471425/80‑1 (MQ=255)   gtgtGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGcccgt                                                                          <  2:471428/81‑3 (MQ=255)   gtgtGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGcccgt                                                                          <  2:471427/81‑3 (MQ=255)    tgtgGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGcccgtg                                                                         >  2:471467/1‑78 (MQ=255)    tgtgGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGcccgtg                                                                         <  2:471429/81‑4 (MQ=255)      tgGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCgtggt                                                                      <  2:471430/81‑1 (MQ=255)        gagaACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCgtggtg                                                                     >  2:471475/1‑80 (MQ=255)                cccGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCtt                                                              <  1:471431/79‑1 (MQ=255)                    aTACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCAc                                                        <  1:471432/81‑1 (MQ=255)                         tCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAAc                                                   >  2:471470/1‑81 (MQ=255)                                 cTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTc                                           <  2:471433/81‑1 (MQ=255)                                      aGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCa                                      >  2:471489/1‑81 (MQ=255)                                       ggCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCAc                                     >  1:471481/1‑81 (MQ=255)                                            cccATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCa                                <  2:471434/81‑1 (MQ=255)                                             ccATCTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg                               <  1:471435/81‑1 (MQ=255)                                                 cTGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCgg                            >  2:471483/1‑80 (MQ=255)                                                  tGCGCCAGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGaa                          >  1:471482/1‑81 (MQ=255)                                                        aGCGGCTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAAcacca                     >  2:471476/1‑80 (MQ=255)                                                             cTTTGACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTgc               <  1:471436/81‑1 (MQ=255)                                                                 gACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGcc            <  1:471439/80‑1 (MQ=255)                                                                 gACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGcc            <  1:471438/80‑1 (MQ=255)                                                                 gACTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGcc            <  1:471437/80‑1 (MQ=255)                                                                   cTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTc         <  1:471442/81‑1 (MQ=255)                                                                   cTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTc         <  1:471440/81‑1 (MQ=255)                                                                   cTGCGGAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTc         <  2:471441/81‑1 (MQ=255)                                                                       ggAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCt     <  1:471443/81‑1 (MQ=255)                                                                       ggAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCt     <  2:471444/81‑1 (MQ=255)                                                                       ggAGAATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCt     >  1:471493/1‑81 (MQ=255)                                                                          gaATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCa  >  2:471486/1‑81 (MQ=255)                                                                          gaATG‑CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCa  <  1:471445/81‑1 (MQ=255)                                                                               |                                                                             GAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCA  >  NC_000913/2898918‑2899072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑