Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,918,157	Δ1 bp	coding (1229/1341 nt)	gudD ←	D‑glucarate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,918,155	0	G	.	100.0%	94.2 / NA	23	coding (1231/1341 nt)	gudD	D‑glucarate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (11/12);  total (11/12)

CCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCTTT  >  NC_000913/2918081‑2918230                                                                           |                                                                            ccagccagGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATggcttt                                                                        >  1:475099/1‑76 (MQ=255)    gccagGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTtcatc                                                                   <  2:475075/81‑1 (MQ=255)    gccagGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTtcat                                                                    >  1:475120/1‑80 (MQ=255)       agGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACt                                                                >  2:475115/1‑81 (MQ=255)        ggAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACtt                                                               <  2:475076/81‑1 (MQ=255)             cAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATc                                                           >  1:475107/1‑80 (MQ=255)              aGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATcc                                                          <  1:475077/80‑1 (MQ=255)                    tGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGa                                                   >  2:475110/1‑81 (MQ=255)                      cATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGa                                                   <  2:475080/79‑1 (MQ=255)                      cATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGAtt                                                 <  2:475078/81‑1 (MQ=255)                      cATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGAt                                                  <  2:475079/80‑1 (MQ=255)                        ttCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTc                                               >  2:475111/1‑81 (MQ=255)                             atcgcatcgTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTacac                                          <  1:475081/81‑1 (MQ=255)                                  atcgTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGc                                     <  1:475082/81‑1 (MQ=255)                                       cACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCgg                                >  2:475109/1‑81 (MQ=255)                                            gcgcCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGttttt                           >  1:475112/1‑81 (MQ=255)                                                     ccGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCt                  <  1:475083/81‑1 (MQ=255)                                                             cTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGc          >  1:475119/1‑81 (MQ=255)                                                               gATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGccc        >  1:475124/1‑81 (MQ=255)                                                               gATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGccc        >  1:475117/1‑81 (MQ=255)                                                                 tACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGc      <  1:475084/81‑1 (MQ=255)                                                                  aCAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGcc     >  1:475106/1‑81 (MQ=255)                                                                    aGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCtt   <  1:475085/81‑1 (MQ=255)                                                                     gCTCAT‑GGCTTTCATCACTTGATCCATATCGAATTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCttt  <  2:475086/81‑1 (MQ=255)                                                                           |                                                                            CCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCTTT  >  NC_000913/2918081‑2918230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑