| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,962,983 | (T)5→4 | coding (174/408 nt) | ygdB ← | DUF2509 domain‑containing protein YgdB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,962,979 | 0 | T | . | 90.5% | 65.2 / 2.7 | 21 | coding (178/408 nt) | ygdB | DUF2509 domain‑containing protein YgdB |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base . (10/9); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
CAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGG > NC_000913/2962899‑2963053 | cAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAt < 2:484260/81‑1 (MQ=255) gTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAttt < 1:484261/81‑1 (MQ=255) gTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAttt > 2:484302/1‑81 (MQ=255) tAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAtttt < 2:484262/81‑1 (MQ=255) aaaCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAttttc > 1:484306/1‑80 (MQ=255) cGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTcccc < 1:484263/81‑1 (MQ=255) gCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTcccca > 1:484305/1‑80 (MQ=255) aaacaaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACg > 2:484299/1‑81 (MQ=255) aacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAgc > 1:484313/1‑81 (MQ=255) ccTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAgcgc > 2:484324/1‑81 (MQ=255) cTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGcc < 2:484264/81‑1 (MQ=255) ggTTTCAGCGTACTGCGAGCACTGAACTGCAGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAAc < 2:484265/80‑1 (MQ=255) ttaGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATg < 2:484266/78‑1 (MQ=255) tCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATgg > 1:484307/1‑81 (MQ=255) gTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg < 1:484267/81‑1 (MQ=255) ctggctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACttt < 1:484268/80‑1 (MQ=255) ctgCGTCTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATg < 1:484269/81‑1 (MQ=255) cTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcg > 2:484308/1‑81 (MQ=255) cTGCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcg > 2:484316/1‑81 (MQ=255) gCCAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACCGAACGATGGCCTGGCGGCCCAATGACTGACTTTCCATGCTCAcgc > 1:484325/1‑80 (MQ=255) ccAGCAGTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCga < 1:484270/81‑1 (MQ=255) cagcagTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcgc > 2:484309/1‑78 (MQ=255) agcagTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCgaga < 2:484271/81‑1 (MQ=255) gcagTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGagag < 2:484272/81‑1 (MQ=255) cagTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAgg > 2:484318/1‑81 (MQ=255) agTGCA‑TTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAgg > 1:484320/1‑80 (MQ=255) | CAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGG > NC_000913/2962899‑2963053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |