Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,967,332	Δ1 bp	coding (1103/2247 nt)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,967,331	0	G	.	100.0%	156.4 / NA	37	coding (1104/2247 nt)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/17);  total (20/17)

AGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTA  >  NC_000913/2967254‑2967407                                                                              |                                                                             aGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACgtag                                                                           <  2:485194/81‑4 (MQ=255)   cAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACgtagg                                                                          >  1:485263/1‑76 (MQ=255)   cAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAggg                                                                        <  1:485195/81‑1 (MQ=255)         aCCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATccc                                                                   <  1:485196/80‑1 (MQ=255)          ccGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATccc                                                                   >  2:485268/1‑79 (MQ=255)           cgcgATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAg                                                                >  2:485250/1‑81 (MQ=255)           cgcgATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAg                                                                >  2:485239/1‑81 (MQ=255)                 aGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACg                                                          >  1:485291/1‑81 (MQ=255)                  gCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACg                                                          >  2:485245/1‑80 (MQ=255)                             tCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCgc                                              <  1:485197/81‑1 (MQ=255)                               aGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCgcag                                            >  1:485261/1‑81 (MQ=255)                                gCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCgcagc                                           <  2:485198/81‑1 (MQ=255)                                  gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCAt                                         >  2:485260/1‑81 (MQ=255)                                  gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCAt                                         >  1:485257/1‑81 (MQ=255)                                  gTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCAt                                         <  1:485199/81‑1 (MQ=255)                                    ccTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATgg                                       >  1:485244/1‑81 (MQ=255)                                     cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATggg                                      <  2:485200/81‑1 (MQ=255)                                     cTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATgg                                       <  1:485201/80‑1 (MQ=255)                                       aCGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAg                                    >  1:485271/1‑81 (MQ=255)                                        cGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGa                                     <  1:485202/79‑1 (MQ=255)                                         gATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGtt                                  >  2:485269/1‑81 (MQ=255)                                         gATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGtt                                  >  1:485253/1‑81 (MQ=255)                                         gATGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGtt                                  >  2:485254/1‑81 (MQ=255)                                          aTGCAGCACCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTg                                 >  1:485262/1‑81 (MQ=255)                                            gcagcaCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGc                               >  2:485256/1‑81 (MQ=255)                                              agcaCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTg                             <  2:485203/81‑1 (MQ=255)                                                 aCCGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTgcgc                          <  1:485204/81‑1 (MQ=255)                                                  ccGAAGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGcc                         <  1:485205/81‑1 (MQ=255)                                                     aaGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCAtc                      >  1:485259/1‑81 (MQ=255)                                                      aGGCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCAtct                     <  1:485206/81‑1 (MQ=255)                                                        gCTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCg                   >  2:485272/1‑81 (MQ=255)                                                         cTGAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGc                  <  1:485207/81‑1 (MQ=255)                                                           gAATATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCAc                >  2:485249/1‑81 (MQ=255)                                                            aaTATCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACg               <  1:485208/81‑1 (MQ=255)                                                             atatCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGa              >  1:485270/1‑81 (MQ=255)                                                              tatCCGCGCCCATCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGac             <  1:485209/81‑1 (MQ=255)                                                                         aTCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACt   >  1:485274/1‑80 (MQ=255)                                                                         aTCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTa  <  2:485210/81‑1 (MQ=255)                                                                         aTCAC‑GTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTa  <  1:485211/81‑1 (MQ=255)                                                                              |                                                                             AGCAATTCACCGCGATAGCCATCAACGATCAGCGTCCTACGATGCAGCACCGAAGGCTGAATATCCGCGCCCATCACGGTAGGGATCCCCAGCGCACGTACCATGATCGCAGCATGGGAGTTGGCTGCGCCATCTCGCACGACAACACCGACTA  >  NC_000913/2967254‑2967407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑