Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,998,759	Δ1 bp	coding (70/795 nt)	ygeQ ←	protein YgeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,998,756	0	C	.	100.0%	108.5 / NA	26	coding (73/795 nt)	ygeQ	protein YgeQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/12);  total (14/12)

TTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGCAGATTTCCCTTTCATA  >  NC_000913/2998678‑2998829                                                                               |                                                                          ttAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTGccc                                                                         >  1:490641/1‑81 (MQ=255)             aaCTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCa                                                            >  2:490644/1‑81 (MQ=255)              aCTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCAt                                                           <  1:490619/81‑1 (MQ=255)                    aTCTGTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGcc                                                      <  2:490619/80‑1 (MQ=255)                        gTTGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTCCCTCAc                                                  >  2:490649/1‑80 (MQ=255)                         ttGCTGCCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCAc                                                  <  2:490620/79‑1 (MQ=255)                          tgctgcCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGa                                                >  2:490642/1‑80 (MQ=255)                              gcCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAata                                           >  2:490656/1‑81 (MQ=255)                              gcCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAata                                           >  2:490647/1‑81 (MQ=255)                              gcCCCCGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAata                                           >  1:490644/1‑81 (MQ=255)                               cccccGTGGAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAatat                                          <  2:490621/81‑1 (MQ=255)                                       gAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCg                                   <  1:490623/80‑1 (MQ=255)                                       gAATTAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCg                                   <  2:490623/80‑1 (MQ=255)                                           tAAGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGTATGTGCCGCACGAAAATATACCAGCGAg                                 >  2:490646/1‑78 (MQ=255)                                            aaGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCag                              >  2:490651/1‑80 (MQ=255)                                            aaGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCag                              <  1:490625/80‑1 (MQ=255)                                             aGAATATTAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCaga                             <  1:490626/80‑1 (MQ=255)                                                    tAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTaa                     >  2:490650/1‑81 (MQ=255)                                                    tAAATACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTaa                     >  2:490655/1‑81 (MQ=255)                                                      aaTACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATg                   <  1:490627/81‑1 (MQ=255)                                                       aTACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGc                  <  2:490628/81‑1 (MQ=255)                                                       aTACAGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGc                  <  1:490628/81‑1 (MQ=255)                                                           aGATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGCAGAt              >  2:490652/1‑81 (MQ=255)                                                            gATTCACTCGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGCAGAtt             >  1:490660/1‑81 (MQ=255)                                                                  cTCGTCACTTCTG‑CCCTGTCGCCTGACATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGCAGATTTCCCtt       <  1:490630/81‑1 (MQ=255)                                                                    cGTCACTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGCAGATTTCCCttt      >  2:490661/1‑80 (MQ=255)                                                                        aCTTCTG‑CCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGCAGATTTCCCTTTCATa  >  2:490653/1‑80 (MQ=255)                                                                               |                                                                          TTAACGATAAATAACTTTTATCTGTTGCTGCCCCCGTGGAATTAAGAATGTTAAATACAGATTCACTCGTCACTTCTGCCCCTGTCGCCTGGCATGTGCCGCACGAAAATATACCAGCGAGCAGAAGAGTTAATGCAGATTTCCCTTTCATA  >  NC_000913/2998678‑2998829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑