Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,006,049	(A)7→6	intergenic (‑263/‑213)	ygeV ← / → ygeW	putative sigma(54)‑dependent transcriptional regulator YgeV/putative carbamoyltransferase YgeW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,006,043	0	A	.	100.0%	63.6 / NA	16	intergenic (‑257/‑219)	ygeV/ygeW	putative sigma(54)‑dependent transcriptional regulator YgeV/putative carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/5);  total (11/5)

GAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAC  >  NC_000913/3005966‑3006106                                                                              |                                                                aaGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATaaa                                                               >  1:492111/2‑80 (MQ=255)  aGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATaaaa                                                              <  2:492093/80‑1 (MQ=255)     ttATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATaaaaaagc                                                          >  2:492112/1‑79 (MQ=255)          aaaCTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCaaa                                                     <  1:492095/80‑1 (MQ=255)           aaCTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACg                                                   >  2:492113/1‑81 (MQ=255)                aaGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTc                                              <  1:492096/81‑1 (MQ=255)                  gagagaTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGt                                            <  1:492097/81‑1 (MQ=255)                      gaTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTAttt                                        >  1:492112/1‑81 (MQ=255)                          gCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTaca                                    <  2:492097/81‑1 (MQ=255)                           ccTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTaca                                    >  2:492116/1‑80 (MQ=255)                                 ccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAaacaac                              >  1:492123/1‑80 (MQ=255)                                      aTCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAg                        >  1:492131/1‑81 (MQ=255)                                      aTCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAg                        >  1:492120/1‑81 (MQ=255)                                       tCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGc                       >  2:492135/1‑81 (MQ=255)                                         ttATTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTg                     >  2:492126/1‑81 (MQ=255)                                           aTTTCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCt                      <  1:492099/78‑1 (MQ=255)                                              tCTGATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCAt                 >  1:492115/1‑80 (MQ=255)                                                 gATACTTAGCAGCAAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAAt             >  2:492118/1‑81 (MQ=255)                                                            gcaAATAAATAACGCGAT‑AAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAc  >  2:492120/1‑81 (MQ=255)                                                                              |                                                                GAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAAC  >  NC_000913/3005966‑3006106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑