Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,058,659	(C)5→4	intergenic (‑249/+7)	serA ← / ← rpiA	phosphoglycerate dehydrogenase/ribose‑5‑phosphate isomerase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,058,655	0	C	.	100.0%	80.5 / NA	20	intergenic (‑245/+11)	serA/rpiA	phosphoglycerate dehydrogenase/ribose‑5‑phosphate isomerase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/12);  total (8/12)

CAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGC  >  NC_000913/3058578‑3058731                                                                              |                                                                             cAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTcccc                                                                           <  2:503376/81‑1 (MQ=255) cAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTcccc                                                                           <  1:503376/81‑1 (MQ=255)      gACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTccccgt                                                                         >  2:503409/1‑76 (MQ=255)         aaTTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATc                                                                  <  1:503378/81‑1 (MQ=255)              ccTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCAttt                                                              <  1:503379/80‑1 (MQ=255)               cTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTca                                                            <  2:503379/81‑1 (MQ=255)                         cGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGtttt                                                  <  2:503380/81‑1 (MQ=255)                             acacATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGaca                                              >  1:503417/1‑81 (MQ=255)                               acaTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACAcc                                            >  1:503420/1‑81 (MQ=255)                               acaTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACAcc                                            >  1:503411/1‑81 (MQ=255)                                  tGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTc                                         <  2:503381/81‑1 (MQ=255)                                  tGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTc                                         <  2:503382/81‑1 (MQ=255)                                           aTTTAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGcc                                <  1:503384/81‑1 (MQ=255)                                            tttAATGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCa                               <  2:503384/81‑1 (MQ=255)                                               aaTGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAAt                             >  1:503414/1‑80 (MQ=255)                                               aaTGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAAt                             >  2:503421/1‑80 (MQ=255)                                                aTGAAGAGAATTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATc                            <  2:503385/80‑1 (MQ=255)                                                         aTTTTTTTAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGt                  <  2:503386/81‑1 (MQ=255)                                                            tttttAACGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCcg               >  1:503415/1‑81 (MQ=255)                                                                  aCGGGGGAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCAc         >  1:503419/1‑81 (MQ=255)                                                                    gggggAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACgg       >  1:503416/1‑81 (MQ=255)                                                                      gggAGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGtt     <  1:503388/81‑1 (MQ=255)                                                                         aGGTT‑CCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGc  <  2:503388/81‑1 (MQ=255)                                                                              |                                                                             CAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGC  >  NC_000913/3058578‑3058731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑