Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,780	Δ1 bp	coding (949/1047 nt)	ansB ←	L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,779	0	C	.	100.0%	138.4 / NA	33	coding (950/1047 nt)	ansB	L‑asparaginase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (18/15);  total (18/15)

GCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGG  >  NC_000913/3099702‑3099851                                                                              |                                                                         gctggatctgctgCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGca                                                                         >  2:512304/1‑78 (MQ=255)    ggatctgctgCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGc                                                                   >  1:512307/1‑81 (MQ=255)     gatctgctgCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGcg                                                                  <  2:512261/81‑1 (MQ=255)       tctgctgCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGcgac                                                                >  2:512311/1‑81 (MQ=255)          gctgcGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGcgacg                                                               >  1:512308/1‑79 (MQ=255)           ctgcGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGcgac                                                                <  1:512264/77‑1 (MQ=255)           ctgcGGATCTTTGGTTAGCGTCAGAGCCAGTTGCAGCAGAAAGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAg                                                            <  1:512263/81‑1 (MQ=255)              cGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGcc                                                          <  2:512265/80‑1 (MQ=255)              cGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCg                                                         <  1:512265/81‑1 (MQ=255)               ggATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGt                                                        <  2:512266/81‑1 (MQ=255)                gATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTa                                                       >  1:512318/1‑81 (MQ=255)                 aTCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTAt                                                      >  1:512324/1‑81 (MQ=255)                   cTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTAttt                                                    >  1:512317/1‑81 (MQ=255)                      tGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTAt                                                      <  1:512268/76‑1 (MQ=255)                        gTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGcat                                               <  2:512268/81‑1 (MQ=255)                            gCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCatcatc                                           >  1:512309/1‑81 (MQ=255)                             cGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCatcatc                                           >  2:512300/1‑80 (MQ=255)                                 agagCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACtt                                      >  2:512325/1‑81 (MQ=255)                                 agagCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACt                                       <  2:512269/80‑1 (MQ=255)                                  gagCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTc                                     >  1:512311/1‑81 (MQ=255)                                   agCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCg                                    >  2:512318/1‑81 (MQ=255)                                    gCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCgg                                   <  2:512270/81‑1 (MQ=255)                                         ttGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATcc                              <  1:512272/81‑1 (MQ=255)                                           gcagcaGAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCt                             <  1:512273/80‑1 (MQ=255)                                                agAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTgg                       <  1:512274/81‑1 (MQ=255)                                                 gAACGCGCGCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGt                      <  2:512274/81‑1 (MQ=255)                                                    cgcgcgCTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAgc                   <  2:512275/81‑1 (MQ=255)                                                     gcgcgcTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAgcg                  >  1:512329/1‑81 (MQ=255)                                                      cgcgcTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAgcgc                 >  2:512321/1‑81 (MQ=255)                                                       gcgcTTTTTGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGcc                >  1:512321/1‑81 (MQ=255)                                                           tttttGCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTc            >  1:512315/1‑81 (MQ=255)                                                                gCGGGTTCAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCTACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTAc       >  1:512316/1‑81 (MQ=255)                                                                       cAGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCggg  >  2:512335/1‑79 (MQ=255)                                                                        aGCGTG‑CAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCggg  >  2:512322/1‑78 (MQ=255)                                                                              |                                                                         GCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGG  >  NC_000913/3099702‑3099851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑