Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,119,442	(A)7→6	intergenic (‑330/+155)	yghJ ← / ← glcA	putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,119,436	0	A	.	100.0%	99.1 / NA	24	intergenic (‑324/+161)	yghJ/glcA	putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/14);  total (10/14)

TAAGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATA  >  NC_000913/3119358‑3119510                                                                               |                                                                           tAAGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaa                                                                           >  2:516483/1‑80 (MQ=255)  aaGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACACAATTAATACAGACTTGTTTTGCGTGATaaa                                                                          >  2:516480/1‑80 (MQ=255)   aGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaa                                                                        <  1:516461/81‑1 (MQ=255)     aTTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaaat                                                                      <  2:516461/81‑2 (MQ=255)      ttGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaaa                                                                       >  2:516486/1‑79 (MQ=255)        gCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATaaaaaatag                                                                    <  1:516463/80‑4 (MQ=255)          aaaaGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATg                                                                >  2:516489/1‑81 (MQ=255)              gCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTtct                                                            >  1:516495/1‑81 (MQ=255)              gCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTtct                                                            <  2:516463/81‑1 (MQ=255)                 aCAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCAc                                                         >  1:516482/1‑81 (MQ=255)                 aCAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCAc                                                         <  2:516464/81‑1 (MQ=255)                     aTAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGctc                                                     <  1:516466/81‑1 (MQ=255)                       aGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGctct                                                    >  2:516491/1‑80 (MQ=255)                        gAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCtt                                                   <  1:516467/80‑1 (MQ=255)                        gAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTa                                                  <  1:516468/81‑1 (MQ=255)                                    cTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAg                                      >  1:516494/1‑81 (MQ=255)                                       atcaAGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGt                                    <  2:516468/80‑1 (MQ=255)                                          aaGCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTAt                                 <  1:516470/80‑1 (MQ=255)                                            gCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCt                               >  2:516495/1‑80 (MQ=255)                                            gCGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTg                              <  1:516471/81‑1 (MQ=255)                                             cGAACATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTg                              >  2:516499/1‑80 (MQ=255)                                                 cATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACg                          >  1:516491/1‑80 (MQ=255)                                                 cATAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGc                         <  2:516471/81‑1 (MQ=255)                                                  aTAATTAATACAGACTTGTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCa                        <  1:516473/81‑1 (MQ=255)                                                                   gTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATtgctgc         <  1:516474/79‑1 (MQ=255)                                                                   gTTTTGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCAt       >  2:516503/1‑81 (MQ=255)                                                                    ttttGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCAt       <  1:516476/80‑1 (MQ=255)                                                                    ttttGCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATg      <  2:516474/81‑1 (MQ=255)                                                                        gCGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGat   >  2:516492/1‑80 (MQ=255)                                                                         cGTGAT‑AAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGata  <  1:516477/80‑1 (MQ=255)                                                                               |                                                                           TAAGATTGCAAAAGCAACAGATAGAACGGTGTTTGCTCATCAAGCGAACATAATTAATACAGACTTGTTTTGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATA  >  NC_000913/3119358‑3119510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑