Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,139,164	Δ1 bp	coding (438/867 nt)	yghU →	disulfide reductase/organic hydroperoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,139,163	0	G	.	100.0%	94.3 / NA	23	coding (437/867 nt)	yghU	disulfide reductase/organic hydroperoxide reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (15/8);  total (15/8)

GATCCTGCTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGG  >  NC_000913/3139086‑3139234                                                                              |                                                                        gATCCTGCTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTgctg                                                                      >  2:520745/1‑78 (MQ=255) gATCCTGCTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTgctg                                                                      >  1:520755/1‑78 (MQ=255) gATCCTGCTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTgct                                                                       <  1:520714/80‑3 (MQ=255)  aTCCTGCTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTgctgt                                                                     >  2:520742/1‑77 (MQ=255)   tCCTGCTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGtt                                                                   <  1:520715/81‑1 (MQ=255)        cTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTgg                                                               <  1:520716/80‑1 (MQ=255)          ttATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGtga                                                            >  1:520751/1‑79 (MQ=255)           tATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGtt                                                             <  1:520717/79‑1 (MQ=255)           tATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGtt                                                             <  2:520717/79‑1 (MQ=255)            aTCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTa                                                            >  2:520748/1‑79 (MQ=255)                 gCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGgcg                                                     >  2:520752/1‑81 (MQ=255)                 gCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGgcg                                                     >  1:520752/1‑81 (MQ=255)                 gCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGgc                                                      <  2:520718/80‑1 (MQ=255)                     agaAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCgg                                                  >  1:520757/1‑80 (MQ=255)                       aaaTTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCAc                                               >  2:520747/1‑81 (MQ=255)                           ttGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGtt                                           >  2:520763/1‑81 (MQ=255)                             ggCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTc                                          <  2:520719/80‑1 (MQ=255)                               cTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTc                                       <  1:520721/81‑1 (MQ=255)                                    tCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTcggcgg                                  >  1:520754/1‑81 (MQ=255)                                        gCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCggtggt                              >  1:520747/1‑81 (MQ=255)                                             aGGATTTGGCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTgg                         >  2:520769/1‑81 (MQ=255)                                                    ggCAAAGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACttt                  >  1:520768/1‑81 (MQ=255)                                                       aaaGCGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTAc               >  2:520773/1‑81 (MQ=255)                                                           cGTACTGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCAt            >  2:520761/1‑80 (MQ=255)                                                                tGAAACGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCa      >  1:520765/1‑81 (MQ=255)                                                                   aaCGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCg   <  1:520722/81‑1 (MQ=255)                                                                     cGATGAACT‑GCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCgg  >  1:520763/1‑80 (MQ=255)                                                                              |                                                                        GATCCTGCTTTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGG  >  NC_000913/3139086‑3139234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑