Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,194,572	(T)6→5	intergenic (+47/+149)	yqiK → / ← sibD	protein YqiK/small RNA SibD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,567	0	T	.	100.0%	149.4 / NA	36	intergenic (+42/+154)	yqiK/sibD	protein YqiK/small RNA SibD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (26/10);  total (26/10)

CCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGG  >  NC_000913/3194491‑3194643                                                                             |                                                                             ccTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                                                          <  1:532610/81‑1 (MQ=255)  cTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttta                                                                         >  1:532648/1‑80 (MQ=255)       caacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCt                                                                   <  1:532611/81‑1 (MQ=255)        aacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTg                                                                  <  2:532611/81‑1 (MQ=255)              cGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTcc                                                            <  1:532613/81‑1 (MQ=255)              cGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTcc                                                            >  2:532662/1‑81 (MQ=255)               gTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCg                                                           >  1:532649/1‑81 (MQ=255)               gTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCg                                                           >  1:532642/1‑81 (MQ=255)               gTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCg                                                           >  1:532647/1‑81 (MQ=255)                       aaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAg                                                   >  1:532654/1‑81 (MQ=255)                       aaaaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAg                                                   >  2:532681/1‑81 (MQ=255)                          aGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTg                                                 >  2:532640/1‑80 (MQ=255)                          aGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTg                                                 >  2:532656/1‑80 (MQ=255)                           gCAGAGTAAACCCTCTTCCAGGAATAATCTCTGGCCCAAAAAGCCGGGGA‑TCTTTATATCTGCGTTCCGCTAAAAAGTGc                                                >  2:532650/1‑80 (MQ=25)                            cAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCa                                               >  1:532650/1‑80 (MQ=255)                             agagTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa                                             >  2:532652/1‑81 (MQ=255)                               agTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTCTATATCTGCGTTCCGCTAAAAAGTGCAAAt                                            >  2:532644/1‑80 (MQ=21)                                  aaaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt                                         >  1:532672/1‑80 (MQ=255)                                   aaCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt                                         >  1:532646/1‑79 (MQ=255)                                    aCCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg                                      <  1:532614/81‑1 (MQ=255)                                         ctTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt                                 >  1:532655/1‑81 (MQ=255)                                            ccAGGAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgtt                                <  1:532615/79‑1 (MQ=255)                                               ggAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc                           <  1:532616/81‑1 (MQ=255)                                                gAATAATCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagca                          <  2:532616/81‑1 (MQ=255)                                                 aataatCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAt                         >  1:532665/1‑81 (MQ=255)                                                     atCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtttt                      <  1:532618/80‑1 (MQ=255)                                                     atCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTg                     >  1:532658/1‑81 (MQ=255)                                                      tCCCTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGc                    <  1:532619/81‑1 (MQ=255)                                                         cTGGCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGt                  >  1:532668/1‑80 (MQ=255)                                                           ggCCCAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGAc               >  1:532664/1‑81 (MQ=255)                                                               cAAAAAGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCt           >  2:532659/1‑81 (MQ=255)                                                                  aaaGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg        >  2:532658/1‑81 (MQ=255)                                                                  aaaGCCGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg        >  2:532672/1‑81 (MQ=255)                                                                      ccGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgggg      >  2:532660/1‑79 (MQ=255)                                                                      ccGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgggg      >  1:532671/1‑79 (MQ=255)                                                                       cGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg   <  2:532619/81‑1 (MQ=255)                                                                       cGGGGA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAg   >  1:532674/1‑81 (MQ=255)                                                                        ggggA‑TTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAgg  >  2:532683/1‑81 (MQ=255)                                                                             |                                                                             CCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGG  >  NC_000913/3194491‑3194643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑