Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,195,223	Δ1 bp	noncoding (18/144 nt)	sibE ←	small RNA SibE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,195,221	0	A	.	100.0%	106.3 / NA	26	noncoding (20/144 nt)	sibE	small RNA SibE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (12/14);  total (12/14)

TGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGC  >  NC_000913/3195142‑3195296                                                                                |                                                                            tgatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGaa                                                                            <  2:532732/81‑1 (MQ=255)           cGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTa                                                                 >  2:532782/1‑81 (MQ=255)            gTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCtt                                                                  <  2:532733/79‑1 (MQ=255)             tcatcaTACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTAcc                                                               >  1:532770/1‑81 (MQ=255)              catcatACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTAccc                                                              >  1:532769/1‑81 (MQ=255)                     cTGGTTGTGTTGTTACTTTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTc                                                        <  2:532734/80‑1 (MQ=25)                        gttgtgttgtTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCtt                                                     >  1:532774/1‑80 (MQ=255)                         ttgtgttgtTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTa                                                   <  1:532736/81‑1 (MQ=255)                            tgttgtTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTAct                                                 <  1:532737/80‑1 (MQ=255)                                         aaGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGaaaaaa                                   >  2:532771/1‑81 (MQ=255)                                         aaGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGaaaaa                                    <  1:532738/80‑1 (MQ=255)                                          aGTTTCCCGACTTACTAACAACTCATCAGAGAGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAaac                                  <  2:532738/81‑1 (MQ=255)                                           gTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGaaaaatc                                  >  2:532766/1‑78 (MQ=255)                                             ttCCCGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAaacaa                                >  2:532779/1‑80 (MQ=255)                                               cccGACTTACTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAg                             >  1:532775/1‑81 (MQ=255)                                                       aCTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAAt                      <  1:532740/80‑1 (MQ=255)                                                        cTAACAACTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAg                    <  1:532741/81‑1 (MQ=255)                                                           acaacTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGc                  <  2:532741/80‑1 (MQ=255)                                                            caacTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt                <  2:532742/81‑1 (MQ=255)                                                            caacTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCt                <  1:532744/81‑1 (MQ=255)                                                               cTCATCAGAGGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGcc             <  2:532744/81‑1 (MQ=255)                                                                      gagGGGGGAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACg      <  2:532745/81‑1 (MQ=255)                                                                        ggggggAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACg      >  2:532777/1‑79 (MQ=255)                                                                        ggggggAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACg      >  2:532781/1‑79 (MQ=255)                                                                          ggggAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGc  <  2:532746/81‑1 (MQ=255)                                                                          ggggAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGc  >  1:532784/1‑81 (MQ=255)                                                                           gggAG‑AATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGc  >  2:532780/1‑80 (MQ=255)                                                                                |                                                                            TGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAAATCCTCCCTTACCCTTGTTCCTTTACTCTAGGTTGAAAAAACAACAGCGTCAATAGGCCTGCCATGTACGAAGC  >  NC_000913/3195142‑3195296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑