Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,268,304	(C)6→5	coding (451/540 nt)	yhaB →	protein YhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,268,299	0	C	.	100.0%	99.4 / NA	24	coding (446/540 nt)	yhaB	protein YhaB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/16);  total (8/16)

ATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTT  >  NC_000913/3268221‑3268374                                                                               |                                                                            aTAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAAccc                                                                           <  2:549410/81‑1 (MQ=255)   aaaaaaGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAAccccc                                                                         >  2:549444/1‑81 (MQ=255)     aaaaGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAAccccctt                                                                       >  2:549438/1‑79 (MQ=255)        agagCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTtgtg                                                                   <  1:549412/81‑1 (MQ=255)           gCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCAc                                                                <  2:549412/81‑1 (MQ=255)                 aTGTATTAAGGGGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTtaat                                                          <  1:549414/81‑1 (MQ=255)                 aTGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTtaa                                                           >  2:549442/1‑80 (MQ=255)                            agaCGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCAt                                               <  2:549414/81‑1 (MQ=255)                               cGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTAc                                            <  2:549415/81‑1 (MQ=255)                                 aTTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACtt                                          <  2:549416/81‑1 (MQ=255)                                     cTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAg                                       <  2:549417/80‑1 (MQ=255)                                        aGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGAttg                                   >  1:549441/1‑81 (MQ=255)                                        aGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGAttg                                   <  2:549418/81‑1 (MQ=255)                                          tATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGAttgtt                                 <  2:549419/81‑1 (MQ=255)                                            ttGTGCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGAttgttg                                <  1:549421/80‑1 (MQ=255)                                              gtgCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGc                              <  2:549421/80‑1 (MQ=255)                                              gtgCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGc                              >  2:549443/1‑80 (MQ=255)                                               tgCAAAATTCACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCa                             <  2:549422/80‑1 (MQ=255)                                                        cACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCttt                   >  2:549448/1‑81 (MQ=255)                                                        cACCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCtt                    <  2:549423/80‑1 (MQ=255)                                                         aCCCGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCCCTTTATTAAACCAGGCATTCCTTCAGATGGTTGCCAATTCCTCtttt                  >  2:549445/1‑81 (MQ=255)                                                            cGAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCttttttt               >  1:549447/1‑81 (MQ=255)                                                             gAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTc              <  1:549426/81‑1 (MQ=255)                                                             gAAAAGAAGCCATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTc              <  1:549425/81‑1 (MQ=255)                                                                      ccATCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCa     <  2:549426/81‑1 (MQ=255)                                                                        aTCTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCAtt   >  1:549455/1‑81 (MQ=255)                                                                          cTTAA‑CCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCAttt  >  2:549455/1‑80 (MQ=255)                                                                               |                                                                            ATAAAAAAGAGCTGGAATGTATTAAGGAGACGATTTCTAAGTATTGTGCAAAATTCACCCGAAAAGAAGCCATCTTAACCCCCCTTGTGCACTTTAATAAAACCAGCATTACTTCAGATTGTTGGCAAATTCTCTTTTTTTCACCCGATCATTT  >  NC_000913/3268221‑3268374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑