Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,283,066	Δ1 bp	coding (1091/1155 nt)	agaS →	putative galactosamine‑6‑phosphate deaminase/isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,283,063	0	C	.	100.0%	107.9 / NA	26	coding (1088/1155 nt)	agaS	putative galactosamine‑6‑phosphate deaminase/isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/12);  total (14/12)

GAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCG  >  NC_000913/3282984‑3283138                                                                                |                                                                            gAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAcc                                                                            >  2:552740/1‑81 (MQ=255) gAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAcc                                                                            >  1:552737/1‑81 (MQ=255)     aGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAcccat                                                                         >  1:552739/1‑78 (MQ=255)      ggCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAcccat                                                                         >  2:552730/1‑77 (MQ=255)         aTTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGc                                                                    >  2:552746/1‑80 (MQ=255)         aTTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGc                                                                    >  1:552754/1‑80 (MQ=255)            ttGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAg                                                                 <  2:552695/80‑1 (MQ=255)                  ccTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCAcc                                                          >  2:552742/1‑81 (MQ=255)                   cTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCAcc                                                          <  1:552697/80‑1 (MQ=255)                      aTGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGt                                                        <  2:552698/79‑1 (MQ=255)                      aTGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTa                                                      <  1:552698/81‑1 (MQ=255)                       tGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTaa                                                     >  2:552754/1‑81 (MQ=255)                        gTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAAc                                                    >  2:552752/1‑81 (MQ=255)                        gTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAAc                                                    >  1:610732/1‑81 (MQ=255)                            gCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACcgcg                                                >  2:552734/1‑81 (MQ=255)                               cAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCgtgg                                             >  1:552758/1‑81 (MQ=255)                               cAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCgtgg                                             <  2:552699/81‑1 (MQ=255)                                 gACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCgtggtg                                           <  1:552701/81‑1 (MQ=255)                                      ttGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAgg                                      >  1:552762/1‑81 (MQ=255)                                        gCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGc                                     <  1:552702/80‑1 (MQ=255)                                           cTGATGCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTaa                                 <  1:552703/81‑1 (MQ=255)                                                gCAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAAtcatt                            >  2:552735/1‑81 (MQ=255)                                                 cAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAAtcattc                           <  1:552705/81‑1 (MQ=255)                                                 cAGTCGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAAtcatt                            <  2:552703/80‑1 (MQ=255)                                                     cGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATcc                       >  2:552744/1‑81 (MQ=255)                                                     cGCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATcc                       >  1:552770/1‑81 (MQ=255)                                                      gCTGCACATGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCg                      >  1:552744/1‑81 (MQ=255)                                                          cacaTGGGCAATACGCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTggc                  <  2:552705/81‑1 (MQ=255)                                                                        gCCGGATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGAt    <  2:552706/81‑1 (MQ=255)                                                                           ggATA‑CCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCg  >  1:552761/1‑80 (MQ=255)                                                                                |                                                                            GAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCG  >  NC_000913/3282984‑3283138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑