Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,354,739	Δ1 bp	coding (15/4461 nt)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,354,737	0	A	.	100.0%	129.4 / NA	31	coding (13/4461 nt)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (15/16);  total (15/16)

TCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCG  >  NC_000913/3354658‑3354813                                                                                |                                                                             tctTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATaa                                                                             >  2:569541/1‑81 (MQ=255)         tgCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCtt                                                                      <  2:569484/79‑1 (MQ=255)          gCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCtt                                                                      >  1:569533/1‑78 (MQ=255)           cccGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTggga                                                                  <  1:569487/81‑1 (MQ=255)           cccGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTgaga                                                                  <  2:569485/81‑1 (MQ=255)            ccGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGagag                                                                 <  1:569488/81‑1 (MQ=255)              gTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAgg                                                                >  1:569547/1‑80 (MQ=255)                 cgcAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGAt                                                             <  2:569489/80‑1 (MQ=255)                 cgcAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATa                                                            <  1:569489/81‑1 (MQ=255)                  gcAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATaa                                                           >  2:569531/1‑81 (MQ=255)                    aGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACt                                                         >  2:569536/1‑81 (MQ=255)                    aGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACt                                                         >  2:569545/1‑81 (MQ=255)                       ggTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACtgtg                                                      >  2:569539/1‑81 (MQ=255)                            ggAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTc                                                 >  1:569544/1‑81 (MQ=255)                            ggAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATAAGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTc                                                 <  2:569490/81‑1 (MQ=255)                                 tGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCt                                            <  1:569492/81‑1 (MQ=255)                                     gTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATc                                        >  1:569551/1‑81 (MQ=255)                                         ccGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGcc                                     <  2:569492/80‑1 (MQ=255)                                          cGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCca                                   >  2:569548/1‑81 (MQ=255)                                           gCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCcac                                  <  1:569494/81‑1 (MQ=255)                                            cAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCcaca                                 >  2:569538/1‑81 (MQ=255)                                            cAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCcaca                                 <  1:569495/81‑1 (MQ=255)                                             agagCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCcaca                                 >  2:569556/1‑80 (MQ=255)                                                  cTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAg                           <  1:569496/81‑1 (MQ=255)                                                        gAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAAc                     <  1:569498/81‑1 (MQ=255)                                                        gAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAAc                     <  2:569496/81‑1 (MQ=255)                                                         aGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAAcc                    <  2:569498/81‑1 (MQ=255)                                                          ggTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAAcc                    >  1:569559/1‑80 (MQ=255)                                                                cGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCcac             <  1:569500/81‑1 (MQ=255)                                                                 gATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCcac             >  2:569547/1‑80 (MQ=255)                                                                   tatGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAg          >  1:569561/1‑81 (MQ=255)                                                                           aCGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCg  >  2:569559/1‑81 (MQ=255)                                                                                |                                                                             TCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCG  >  NC_000913/3354658‑3354813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑