Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,667,617	(A)6→5	intergenic (‑37/+174)	gadA ← / ← ccp	glutamate decarboxylase A/cytochrome c peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,667,612	0	A	.	100.0%	94.4 / NA	23	intergenic (‑32/+179)	gadA/ccp	glutamate decarboxylase A/cytochrome c peroxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (7/16);  total (7/16)

GAATCGAGTAGTTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAATAAAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGCAGAAAAA  >  NC_000913/3667534‑3667688                                                                               |                                                                             gAATCGAGTAGTTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAATaa                                                                             >  2:645923/1‑80 (MQ=255)   aTCGAGTAGTTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAATaaaaa                                                                          <  1:645883/81‑1 (MQ=255)         tagtTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGa                                                                   <  1:645884/81‑1 (MQ=255)           gtTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGAtt                                                                 >  2:645926/1‑81 (MQ=255)           gtTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGAtt                                                                 <  2:645884/81‑1 (MQ=255)           gtTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGAt                                                                  <  2:645886/80‑1 (MQ=255)           gtTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGAt                                                                  <  2:645885/80‑1 (MQ=255)                  gCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGc                                                          <  1:645889/81‑1 (MQ=255)                  gCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGc                                                          <  2:645887/81‑1 (MQ=255)                   cGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCa                                                         <  2:645889/81‑1 (MQ=255)                       aaTCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATgg                                                     <  2:645890/81‑1 (MQ=255)                           cGTTAACAGCTTCTGGTCCATTGCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAg                                                  <  1:645892/80‑1 (MQ=39)                             ttAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGc                                                 >  1:645926/1‑79 (MQ=255)                               aaCAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAgg                                             >  1:645925/1‑81 (MQ=255)                               aaCAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAg                                              <  1:645893/80‑1 (MQ=255)                                   gCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCAtt                                         <  2:645893/81‑1 (MQ=255)                                       cTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTAc                                       <  2:645894/79‑1 (MQ=255)                                                 tCGAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAAttt                            <  2:645895/80‑1 (MQ=255)                                                   gAACTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTaa                          <  2:645896/80‑1 (MQ=255)                                                     aCTCCTTAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAAt                        >  1:645939/1‑80 (MQ=255)                                                          ttAAATTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACaa                   >  2:645922/1‑80 (MQ=255)                                                            aaaTTTATTTGAAGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACaaa                  <  2:645897/79‑1 (MQ=255)                                                                       aaGGCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGCAGa      >  1:645928/1‑80 (MQ=255)                                                                       aaGGCAAT‑AAAAAGTAGGATTTATCCGCAATAGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGCAGaa     <  2:645898/81‑1 (MQ=255)                                                                          gCAAT‑AAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGCAGaaaaa  >  1:645932/1‑81 (MQ=255)                                                                               |                                                                             GAATCGAGTAGTTCTGAGCGGAAATCCGTTAACAGCTTCTGGTCCATTTCGAACTCCTTAAATTTATTTGAAGGCAATAAAAAAGTAGGATTTATCCGCAATGGAAGCAAGGCATTACAGGCTTAATTTAAATAACAAAATCCTAAGCAGAAAAA  >  NC_000913/3667534‑3667688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑