Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,710,634	Δ1 bp	intergenic (‑159/+165)	eptB ← / ← yhjX	Kdo2‑lipid A phosphoethanolamine 7''‑transferase/putative pyruvate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,710,633	0	A	.	100.0%	143.4 / NA	34	intergenic (‑158/+166)	eptB/yhjX	Kdo2‑lipid A phosphoethanolamine 7''‑transferase/putative pyruvate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (19/15);  total (19/15)

TCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAAT  >  NC_000913/3710557‑3710708                                                                             |                                                                            tCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGagcac                                                                         <  1:657434/81‑5 (MQ=255)  cAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGagcac                                                                         >  2:657466/1‑76 (MQ=255)   aGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGcaca                                                                       <  2:657434/80‑1 (MQ=255)     aTAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAAt                                                                    >  2:657471/1‑81 (MQ=255)       aaGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAAtt                                                                   >  1:657494/1‑80 (MQ=255)          tAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACa                                                               <  1:657436/81‑1 (MQ=255)            aaaTCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACaa                                                              >  2:657468/1‑80 (MQ=255)                  acacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGc                                                        >  1:657482/1‑80 (MQ=255)                   cacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAAt                                                          <  2:657436/77‑1 (MQ=255)                   cacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGc                                                        >  2:657477/1‑79 (MQ=255)                   cacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCaa                                                      <  1:657439/81‑1 (MQ=255)                   cacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCa                                                       <  2:657437/80‑1 (MQ=255)                    acGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAg                                                     <  1:657440/81‑1 (MQ=255)                        gcgAATAAGTAGACCACAGCAAAGGGAAAAAATGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTg                                                 <  2:657440/81‑1 (MQ=255)                             tAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAag                                            <  2:657441/81‑1 (MQ=255)                              aaGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGaaaa                                             >  2:657475/1‑79 (MQ=255)                              aaGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGaaaa                                             >  2:657472/1‑79 (MQ=255)                               aGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAagag                                          <  2:657442/81‑1 (MQ=255)                                 tAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGAc                                        <  2:657443/81‑1 (MQ=255)                                      cacaGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAg                                   >  2:657495/1‑81 (MQ=255)                                        caGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCg                                 >  2:657489/1‑81 (MQ=255)                                        caGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCg                                 >  1:657484/1‑81 (MQ=255)                                           cAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTa                              >  2:657478/1‑81 (MQ=255)                                                ggAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACaaa                          >  1:657475/1‑80 (MQ=255)                                                 gAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACaaa                          >  2:657473/1‑79 (MQ=255)                                                 gAAAAAGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACaaa                          >  2:657479/1‑79 (MQ=255)                                                   aaaaGTGTGGGGAAAGAGTGAGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGc                      <  2:657444/81‑1 (MQ=255)                                                    aaaGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAg                       >  1:657499/1‑79 (MQ=255)                                                     aaGTGTGGGGAAAGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAg                    >  2:657486/1‑81 (MQ=255)                                                                aaGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGCGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGcc         <  2:657446/81‑1 (MQ=255)                                                                aaGAGTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGcc         <  1:657446/81‑1 (MQ=255)                                                                   agTGTGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGt      >  1:657495/1‑81 (MQ=255)                                                                    gtgtGCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGt      >  1:657491/1‑80 (MQ=255)                                                                     tgtgCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTa    <  1:657448/81‑1 (MQ=255)                                                                      gtgCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTaa   >  1:657483/1‑81 (MQ=255)                                                                       tgCATG‑AGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAAt  <  2:657448/81‑1 (MQ=255)                                                                             |                                                                            TCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAAT  >  NC_000913/3710557‑3710708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑