Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,719,397	Δ1 bp	intergenic (‑353/‑81)	yiaF ← / → yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,719,394	0	C	.	100.0%	107.2 / NA	26	intergenic (‑350/‑84)	yiaF/yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/14);  total (12/14)

TATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTT  >  NC_000913/3719315‑3719465                                                                                |                                                                        tATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCAcc                                                                        >  2:659735/1‑81 (MQ=255)  aTCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCAcc                                                                        <  1:659693/80‑1 (MQ=255)   tCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCAccc                                                                       <  1:659694/80‑1 (MQ=255)   tCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCAccc                                                                       <  2:659695/80‑1 (MQ=255)   tCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCAccc                                                                       <  1:659695/80‑1 (MQ=255)       aTCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCA‑CCCGCtgt                                                                 >  2:659731/1‑81 (MQ=255)       aTCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCA‑CCCGCtgt                                                                 <  2:659696/81‑1 (MQ=255)         cGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTgtgt                                                               >  1:659744/1‑81 (MQ=255)           ttAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCt                                                             <  2:659697/81‑1 (MQ=255)                     ataAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCA‑TCCGCTGTGTCTGCTTTTccc                                                    >  1:659742/1‑80 (MQ=255)                        aGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACt                                                <  1:659699/81‑1 (MQ=255)                             cGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCt                                           <  2:659699/81‑1 (MQ=255)                                         ggCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCg                               <  2:659700/81‑1 (MQ=255)                                              aatcgaatcATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGc                           >  1:659750/1‑80 (MQ=255)                                               atcgaatcATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTCTCCCGACTATTCTTAATGAGCTTCGATGCaa                         <  1:659702/81‑1 (MQ=255)                                                tcgaatcATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAAt                        <  1:659703/81‑1 (MQ=255)                                                tcgaatcATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAAt                        >  2:659744/1‑81 (MQ=255)                                                  gaatcATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAAtt                       >  1:659739/1‑80 (MQ=255)                                                   aatcATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCa                     <  1:659704/81‑1 (MQ=255)                                                    atcATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCAc                    <  1:659705/81‑1 (MQ=255)                                                     tcATAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACg                   <  1:659706/81‑1 (MQ=255)                                                       aTAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGAt                 >  1:659740/1‑81 (MQ=255)                                                       aTAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGAt                 <  2:659706/81‑1 (MQ=255)                                                       aTAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGAt                 >  2:659742/1‑81 (MQ=255)                                                       aTAGCCAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGAt                 >  2:659757/1‑81 (MQ=255)                                                            cAGAGCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGc            <  2:659707/81‑1 (MQ=255)                                                              gagCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAg          >  2:659750/1‑81 (MQ=255)                                                               agCATGCCCTGACTTCA‑CCCGCTGTGTCTGCTTTTCCAGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAgt         >  2:659747/1‑81 (MQ=255)                                                                     cccTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGAt    <  2:659708/80‑1 (MQ=255)                                                                      ccTGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGAttt  <  2:659709/81‑1 (MQ=255)                                                                        tGACTTCA‑CCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGAttt  >  1:659747/1‑79 (MQ=255)                                                                                |                                                                        TATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTT  >  NC_000913/3719315‑3719465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑