Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,778,242	(G)5→4	coding (844/1656 nt)	lldP →	lactate/glycolate:H(+) symporter LldP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,778,238	0	G	.	100.0%	155.9 / NA	37	coding (840/1656 nt)	lldP	lactate/glycolate:H(+) symporter LldP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/19);  total (18/19)

CTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTGGGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTGCCTGGACACCG  >  NC_000913/3778159‑3778313                                                                                |                                                                            ctTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTgg                                                                            >  2:674739/1‑81 (MQ=255)    cGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTgggg                                                                          <  2:674682/80‑1 (MQ=255)    cGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTggg                                                                           <  2:674683/79‑1 (MQ=255)         gTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTcac                                                                   <  1:674686/81‑1 (MQ=255)         gTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTca                                                                    <  1:674685/80‑1 (MQ=255)            tCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAg                                                                 <  2:674688/80‑1 (MQ=255)               cTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGtt                                                              >  1:674736/1‑80 (MQ=255)               cTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTg                                                             <  2:674689/81‑1 (MQ=255)                tGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGa                                                            >  1:674741/1‑81 (MQ=255)                  ctctGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGata                                                          >  1:674735/1‑81 (MQ=255)                     tGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGatat                                                         <  2:674690/79‑1 (MQ=255)                       ccTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACg                                                     <  2:674691/81‑1 (MQ=255)                       ccTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACg                                                     >  1:674739/1‑81 (MQ=255)                          gACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTg                                                  >  2:674751/1‑81 (MQ=255)                           aCGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTgg                                                 >  2:674742/1‑81 (MQ=255)                             gCTGTTCCTCAGACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGcc                                               <  1:674694/81‑1 (MQ=255)                             gCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGcc                                               <  2:674692/81‑1 (MQ=255)                              cTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGccc                                              <  2:674694/81‑1 (MQ=255)                                gTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCca                                             >  2:674745/1‑80 (MQ=255)                                 ttCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCcaca                                           <  2:674695/81‑1 (MQ=255)                                   ccTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACAcc                                         >  2:674754/1‑81 (MQ=255)                                      cAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGt                                      >  2:674760/1‑81 (MQ=255)                                       aaaCGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGt                                      >  2:674743/1‑80 (MQ=255)                                         aCGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTac                                   <  2:674697/81‑1 (MQ=255)                                         aCGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTa                                    >  2:674757/1‑80 (MQ=255)                                         aCGCTGGCAGCCAGTGCGTGTATTCCGTTTTAGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTac                                   <  2:674696/81‑1 (MQ=255)                                            cTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACt                                <  1:674699/81‑1 (MQ=255)                                                  gCCAGTGCGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGt                          >  2:674753/1‑81 (MQ=255)                                                         cGTGTATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGgtg                    <  1:674700/80‑1 (MQ=255)                                                          gtgtATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGgtgt                   >  2:674765/1‑80 (MQ=255)                                                          gtgtATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGgtgt                   >  1:674749/1‑80 (MQ=255)                                                          gtgtATTCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGtt                  <  2:674700/81‑1 (MQ=255)                                                               ttCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGt              <  1:674702/80‑1 (MQ=255)                                                               ttCCGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTg             >  2:674750/1‑81 (MQ=255)                                                                 ccGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTGcc           >  1:674747/1‑81 (MQ=255)                                                                 ccGTTTTGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTGc            <  1:674703/80‑1 (MQ=255)                                                                    ttttGGTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTGCCTg         >  2:674764/1‑80 (MQ=255)                                                                         gTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTGCCTGGACAcc   <  2:674703/81‑1 (MQ=255)                                                                         gTGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTGCCTGGACAcc   <  1:674705/81‑1 (MQ=255)                                                                          tGATTT‑GGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTGCCTGGACACCg  >  1:674776/1‑81 (MQ=255)                                                                                |                                                                            CTTCGCTGGTATCACTGCTCTGCCTGACGCTGTTCCTCAAACGCTGGCAGCCAGTGCGTGTATTCCGTTTTGGTGATTTGGGGGCGTCACAGGTTGATATGACGCTGGCCCACACCGGTTACACTGCGGGTCAGGTGTTACGTGCCTGGACACCG  >  NC_000913/3778159‑3778313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑