Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,797,159	(A)6→5	coding (212/1260 nt)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,154	0	A	.	100.0%	32.7 / NA	9	coding (207/1260 nt)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/4);  total (5/4)

CATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAG  >  NC_000913/3797076‑3797227                                                                               |                                                                          cATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATtataatataa                                                                          >  2:679824/1‑80 (MQ=255)         tatataATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTAt                                                                >  2:679836/1‑81 (MQ=255)           tataATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTAttc                                                              <  2:679814/81‑1 (MQ=255)                             gTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATAttt                                             <  1:679816/80‑1 (MQ=255)                             gTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATAttt                                             <  1:679817/80‑1 (MQ=255)                                tGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTa                                          <  1:679818/80‑1 (MQ=255)                                     aTTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGgc                                    >  2:679833/1‑81 (MQ=255)                                              aaTTTTACGTGGCAGACAAGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGAt                           >  1:679833/1‑81 (MQ=255)                                                               aaGAAAATTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGTTTTAATTTGGTATTCTGc          >  1:679845/1‑81 (MQ=255)                                                                   aaaTTATAATAT‑AAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGtt       >  1:679836/1‑80 (MQ=255)                                                                        ataatat‑aaAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAg  <  1:679819/80‑1 (MQ=255)                                                                          aatat‑aaAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAg  >  1:679841/1‑78 (MQ=255)                                                                               |                                                                          CATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAG  >  NC_000913/3797076‑3797227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑