Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,949,783	(T)7→6	coding (187/1521 nt)	yifB ←	putative magnesium chelatase YifB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,949,777	0	T	.	100.0%	150.0 / NA	36	coding (193/1521 nt)	yifB	putative magnesium chelatase YifB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/17);  total (19/17)

AGCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGATTTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCGTTGTT  >  NC_000913/3949701‑3949853                                                                             |                                                                             aGCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGAttttt                                                                          >  2:720481/1‑81 (MQ=255)  gCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGAtttttt                                                                         <  2:720434/81‑1 (MQ=255)  gCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGAtttttt                                                                         <  1:720436/81‑1 (MQ=255)      cGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGAttttttcgcc                                                                     <  1:720437/81‑5 (MQ=255)       gCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCg                                                                   >  1:720485/1‑81 (MQ=255)             gCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATAtt                                                             <  1:720438/81‑1 (MQ=255)              cGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTc                                                            <  1:720439/81‑1 (MQ=255)               gATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCa                                                           >  2:720482/1‑81 (MQ=255)                 tAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCata                                                         >  1:720481/1‑81 (MQ=255)                 tAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCata                                                         <  1:720440/81‑1 (MQ=255)                   ggTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATc                                                       <  2:720440/81‑1 (MQ=255)                      aaaTCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGc                                                    >  1:720498/1‑81 (MQ=255)                                gTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAAt                                          >  2:720483/1‑81 (MQ=255)                                gTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAAt                                          >  2:720490/1‑81 (MQ=255)                                 tCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATg                                         <  2:720441/81‑1 (MQ=255)                                   ccccTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGgc                                       <  1:720443/81‑1 (MQ=255)                                   ccccTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGgc                                       <  1:720444/81‑1 (MQ=255)                                      cttcttTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCt                                    <  1:720445/81‑1 (MQ=255)                                            ttGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTgcg                                 <  2:720445/78‑1 (MQ=255)                                            ttGGCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCAc                              >  2:720494/1‑81 (MQ=255)                                               gCAGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCAcgc                            <  1:720447/80‑1 (MQ=255)                                                 aGATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGAt                         <  1:720448/81‑1 (MQ=255)                                                  gATCAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATc                        >  1:720500/1‑81 (MQ=255)                                                  gATCAGCTGGAGCCAGGTCGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATc                        <  2:720448/81‑1 (MQ=255)                                                     cAGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATcgc                      >  1:720488/1‑80 (MQ=255)                                                      aGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATcgcg                     <  1:720450/80‑1 (MQ=255)                                                      aGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATcgc                      >  1:720490/1‑79 (MQ=255)                                                      aGCTGGAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATcgc                      >  2:720485/1‑79 (MQ=255)                                                          ggAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGctt                >  2:720491/1‑81 (MQ=255)                                                          ggAGCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGctt                >  2:720492/1‑81 (MQ=255)                                                             gCCAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGcttctt             >  1:720499/1‑81 (MQ=255)                                                              ccAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCttt            <  1:720451/81‑1 (MQ=255)                                                               cAGGTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCtttt           <  2:720451/81‑1 (MQ=255)                                                                 ggTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTAc         <  1:720453/81‑1 (MQ=255)                                                                 ggTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTAc         >  2:720496/1‑81 (MQ=255)                                                                  gTTGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTAcc        >  1:720489/1‑81 (MQ=255)                                                                   ttGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCg       >  2:720501/1‑81 (MQ=255)                                                                    tGATGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCgt      >  1:720501/1‑81 (MQ=255)                                                                      aTGGTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCgtt     <  2:720453/80‑1 (MQ=255)                                                                        ggTGA‑TTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCgttgtt  >  1:720494/1‑81 (MQ=255)                                                                             |                                                                             AGCAACGCAATGGCGATAGGTAAATCATATCGTCCCCCTTCTTTTGGCAGATCAGCTGGAGCCAGGTTGATGGTGATTTTTTTCGCCGGATATTCATATCCGCTATTGATAATGGCGCTGCGCACGCGATCGCGAGCTTCTTTTACCGTTGTT  >  NC_000913/3949701‑3949853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑