Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,819	(T)6→5	coding (181/1221 nt)	sbmA →	peptide antibiotic/peptide nucleic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,814	0	T	.	100.0%	68.2 / NA	17	coding (176/1221 nt)	sbmA	peptide antibiotic/peptide nucleic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/9);  total (8/9)

ACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGCATCGTTG  >  NC_000913/396736‑396886                                                                               |                                                                         aCTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttt                                                                        <  1:81160/81‑1 (MQ=255)     ggTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttttta                                                                     <  1:81163/80‑2 (MQ=255)     ggTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttttt                                                                      <  2:81162/79‑1 (MQ=255)     ggTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGAttttt                                                                      <  2:81161/79‑1 (MQ=255)        ggCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCt                                                                >  1:81205/1‑81 (MQ=255)            cgTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTtact                                                            <  1:81164/81‑1 (MQ=255)                  aCCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTg                                                      <  2:81165/81‑1 (MQ=255)                       cgcTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGc                                                 >  2:81212/1‑81 (MQ=255)                         cTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCg                                                >  2:81204/1‑80 (MQ=255)                            ccGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAg                                             >  1:81196/1‑80 (MQ=255)                             cGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGa                                           <  1:81166/81‑1 (MQ=255)                                gCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACtt                                        >  2:81202/1‑81 (MQ=255)                                   gATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACttttt                                     <  1:81167/81‑1 (MQ=255)                                              gcgcCGCGCGTTTCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCt                           <  1:81168/80‑1 (MQ=255)                                                          tCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAg              >  1:81201/1‑81 (MQ=255)                                                          tCTGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAg              >  1:81206/1‑81 (MQ=255)                                                            tGGTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGcc            >  1:81203/1‑81 (MQ=255)                                                              gTCGTTGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGcc            <  2:81169/79‑1 (MQ=255)                                                                  ttGGATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTTTCTACAGCCCGCATc      <  1:81170/81‑1 (MQ=255)                                                                     gATTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGCATCGt    <  1:81171/80‑1 (MQ=255)                                                                      aTTTCCTGA‑TTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGCATCGTTg  <  2:81172/81‑1 (MQ=255)                                                                               |                                                                         ACTGGGTGGCGCGTATCACCGGCGCTTCCGGGCAGATCCCGATTAGCGCCGCGCGTTTCTGGTCGTTGGATTTCCTGATTTTTTACGCTTACTACATTGTTTGCGTAGGACTTTTTGCATTGTTCTGGTTTATCTACAGCCCGCATCGTTG  >  NC_000913/396736‑396886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑