Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,333,425	Δ1 bp	coding (526/669 nt)	basR ←	DNA‑binding transcriptional dual regulator BasR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,333,424	0	T	.	100.0%	166.2 / NA	39	coding (527/669 nt)	basR	DNA‑binding transcriptional dual regulator BasR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/25);  total (14/25)

GCCCACTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTGGGCGT  >  NC_000913/4333345‑4333500                                                                                |                                                                             gCCCACTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGtg                                                                             >  2:822493/1‑80 (MQ=255)  cccACTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGtgt                                                                            <  1:822430/81‑3 (MQ=255)   ccACTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGtgta                                                                           <  2:822430/81‑4 (MQ=255)    cACTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGtgtag                                                                          <  1:822432/81‑5 (MQ=255)      cTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTagag                                                                       <  2:822432/81‑1 (MQ=255)          gTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAAtt                                                                   >  1:822490/1‑81 (MQ=255)              cgcAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTccc                                                               <  1:822434/81‑1 (MQ=255)                cAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGa                                                             <  2:822434/81‑1 (MQ=255)                 aGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGAt                                                            <  2:822435/81‑1 (MQ=255)                 aGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGAt                                                            <  2:822436/81‑1 (MQ=255)                 aGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGAt                                                            <  2:822437/81‑1 (MQ=255)                 aGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGAt                                                            >  1:822497/1‑81 (MQ=255)                  gATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATg                                                           <  2:822438/81‑1 (MQ=255)                      gtgGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCAc                                                        <  1:822440/80‑1 (MQ=255)                      gtgGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCAc                                                        <  2:822440/80‑1 (MQ=255)                           tatGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGAct                                                  <  2:822441/81‑1 (MQ=255)                            atGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGActg                                                 >  2:822497/1‑81 (MQ=255)                              gCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGActgcc                                               >  1:822500/1‑81 (MQ=255)                               cACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGActgcct                                              <  2:822442/81‑1 (MQ=255)                               cACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGActgcc                                               <  1:822444/80‑1 (MQ=255)                                aCTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGActgcctg                                             >  2:822518/1‑81 (MQ=255)                                aCTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGActgcctg                                             >  1:822538/1‑81 (MQ=255)                                 cTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGActgcctgc                                            <  2:822444/81‑1 (MQ=255)                                    ccAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCttt                                         >  1:822499/1‑81 (MQ=255)                                     cAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCttt                                         <  2:822446/80‑1 (MQ=255)                                     cAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTg                                        <  2:822445/81‑1 (MQ=255)                                        ggTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGc                                     <  1:822448/81‑1 (MQ=255)                                            ttGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCAtt                                  >  1:822511/1‑80 (MQ=255)                                              ggTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAAc                               >  2:822504/1‑81 (MQ=255)                                              ggTCGAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAAc                               >  2:822514/1‑81 (MQ=255)                                                  gAGGGTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTg                           <  2:822448/81‑1 (MQ=255)                                                    gggTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAc                         <  1:822450/81‑1 (MQ=255)                                                     ggTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAca                        <  2:822450/81‑1 (MQ=255)                                                     ggTTCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAca                        <  2:822451/81‑1 (MQ=255)                                                       ttCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAcagc                      <  2:822452/81‑1 (MQ=255)                                                       ttCATTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAcag                       >  1:822506/1‑80 (MQ=255)                                                          aTTGTCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGAcagca                     >  1:822502/1‑79 (MQ=255)                                                              tCCCAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCat               <  1:822454/81‑1 (MQ=255)                                                                 cAGTTATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCatagt            <  1:822455/81‑3 (MQ=255)                                                                    ttATAGATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCtt         >  1:822501/1‑81 (MQ=255)                                                                         gATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTggg     <  1:822457/80‑1 (MQ=255)                                                                         gATGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTGGGc    <  1:822456/81‑1 (MQ=255)                                                                           tGTCG‑TGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTGGGCGt  >  1:822508/1‑81 (MQ=255)                                                                                |                                                                             GCCCACTTTGTCGCGCAGATTGTGGATATGCACTTCCAGGGTGTTGGTCGAGGGTTCATTGTCCCAGTTATAGATGTCGTTGTAGAGAATTTCCCGATGCACCGGACTGCCTGCTTTGAGCATTAACCGTGACAGCAGAGCATATTCTTTGGGCGT  >  NC_000913/4333345‑4333500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑