Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,342,680	(A)5→4	coding (770/1356 nt)	melA →	alpha‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,342,676	0	A	.	96.7%	116.8 / ‑2.4	30	coding (766/1356 nt)	melA	alpha‑galactosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (13/16);  minor base T (1/0);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAG  >  NC_000913/4342597‑4342748                                                                                |                                                                         ttATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCa                                                                          >  2:824610/1‑80 (MQ=255)      aaGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCaaaagct                                                                    >  2:824623/1‑78 (MQ=255)          agggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGc                                                               <  1:824578/81‑1 (MQ=255)          agggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGc                                                               <  2:824576/81‑1 (MQ=255)           gggcaggCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTT‑TAAAAGCTGGGc                                                               >  1:824635/1‑80 (MQ=255)              caggcaCCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTAtt                                                           <  2:824578/81‑1 (MQ=255)                    ccGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTTCGCTACTAAATGTTC‑AAAAGCTGGGCTATTTCGtaa                                                     >  2:824628/1‑79 (MQ=255)                      gAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACg                                                   >  2:824625/1‑81 (MQ=255)                       aaGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACgg                                                  <  2:824579/81‑1 (MQ=255)                       aaGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACg                                                   >  1:824623/1‑80 (MQ=255)                           cGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATc                                              <  1:824581/81‑1 (MQ=255)                            gAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCg                                             >  2:824632/1‑81 (MQ=255)                              atatTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTc                                           <  2:824581/81‑1 (MQ=255)                                 ttCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAg                                         <  2:824582/80‑1 (MQ=255)                                      ggCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACAtt                                   >  2:824616/1‑81 (MQ=255)                                       gCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACAttt                                  <  2:824584/81‑1 (MQ=255)                                       gCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACAtt                                   <  2:824583/80‑1 (MQ=255)                                        cAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACAtttt                                 <  1:824586/81‑1 (MQ=255)                                           tACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCt                              >  1:824638/1‑81 (MQ=255)                                             cTCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCt                              >  2:824649/1‑79 (MQ=255)                                              tCGCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAg                           >  2:824638/1‑81 (MQ=255)                                                gCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGt                          <  1:824587/80‑1 (MQ=255)                                                gCTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTa                         >  1:824625/1‑81 (MQ=255)                                                 cTGCCAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTac                        <  2:824587/81‑1 (MQ=255)                                                    ccAGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTacaca                     <  2:824588/81‑1 (MQ=255)                                                      aGAATATTGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACAcc                   >  1:824643/1‑81 (MQ=255)                                                            ttGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGtt             <  2:824589/81‑1 (MQ=255)                                                            ttGTGCGCTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGtt             >  1:824641/1‑81 (MQ=255)                                                                 cgcTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGGATCGTCAGAACATTTTGCTGAGTACACACCGTGGTttatt         <  1:824592/80‑1 (MQ=255)                                                                 cgcTACGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTttatt         <  2:824590/80‑1 (MQ=255)                                                                      cGAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATAGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGc     >  2:824630/1‑79 (MQ=255)                                                                       gAAATGTTC‑AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAg  >  2:824646/1‑81 (MQ=255)                                                                                |                                                                         TTATGAAGCAGGGCAGGCACCGAAGCCGAATATTCATGGCAATACTCGCTGCCAGAATATTGTGCGCTACGAAATGTTCAAAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAG  >  NC_000913/4342597‑4342748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑