Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	408,476	Δ1 bp	intergenic (+15/‑133)	ppnP → / → ykiA	nucleoside phosphorylase PpnP/DUF2773 domain‑containing protein YkiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	408,473	0	C	.	100.0%	107.3 / NA	26	intergenic (+12/‑136)	ppnP/ykiA	nucleoside phosphorylase PpnP/DUF2773 domain‑containing protein YkiA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (7/19);  total (7/19)

ACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGATTT  >  NC_000913/408403‑408546                                                                       |                                                                          acaGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACg                                                                 <  2:83275/80‑1 (MQ=255)  caGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACggg                                                               <  1:83276/81‑1 (MQ=255)  caGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACggg                                                               <  2:83277/81‑1 (MQ=255)   aGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCTGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGa                                                              <  1:83278/81‑1 (MQ=255)   aGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACgg                                                                >  2:83308/1‑79 (MQ=255)    gTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAg                                                             <  2:83279/81‑1 (MQ=255)    gTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAg                                                             <  1:83280/81‑1 (MQ=255)             aTCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCAttttt                                                    <  2:83281/81‑1 (MQ=255)             aTCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCAttttt                                                    >  2:83301/1‑81 (MQ=255)                  cAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCt                                                  <  1:83282/78‑1 (MQ=255)                    aGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAat                                             <  2:83283/81‑1 (MQ=255)                     gTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAata                                            >  2:83305/1‑81 (MQ=255)                          cGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCctt                                       <  2:83284/81‑1 (MQ=255)                           gAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCcttt                                      <  2:83285/81‑1 (MQ=255)                            aaCCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCctttc                                     >  1:83312/1‑81 (MQ=255)                            aaCCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCctttc                                     <  1:83286/81‑1 (MQ=255)                                      ttATCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGccc                             <  1:83287/79‑1 (MQ=255)                                        aTCTGTGCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCat                           <  2:83288/79‑1 (MQ=255)                                          cTGTGCAGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCataat                        >  1:83314/1‑80 (MQ=255)                                             tgCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCataataata                    >  2:83315/1‑81 (MQ=255)                                             tgCCGCTATCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCataataa                      <  1:83289/79‑1 (MQ=255)                                                     tCTGTAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCt              <  2:83290/79‑1 (MQ=255)                                                         tAATTCCTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTtgct         <  1:83291/80‑1 (MQ=255)                                                           aTTCCTCGCCTT‑CACTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAGTAATATTTCCTTtgctgc       <  2:83292/80‑1 (MQ=255)                                                               cTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGAttt  >  1:83313/1‑81 (MQ=255)                                                               cTCGCCTT‑CCCTTGAACGGGAGGGCATTTTTCTGAAACATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGAtt   <  2:83293/80‑1 (MQ=255)                                                                       |                                                                          ACAGTGAGTTTCATCTGCAAGTTGCCGAACCCACCTCTTATCTGTGCCGCTATCTGTAATTCCTCGCCTTCCCCTTGAACGGGAGGGCATTTTTCTGAAATATCCTTTCTTTAGCCCATAATAATATTTCCTTTGCTGCGATTT  >  NC_000913/408403‑408546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑