Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	451,589	Δ1 bp	coding (22/948 nt)	cyoA ←	cytochrome bo3 ubiquinol oxidase subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	451,586	0	T	.	100.0%	89.5 / NA	22	coding (25/948 nt)	cyoA	cytochrome bo3 ubiquinol oxidase subunit 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/14);  total (8/14)

GTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGC  >  NC_000913/451508‑451659                                                                               |                                                                          gTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCTCAAACtt                                                                          >  2:91701/1‑80 (MQ=255)    cTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACtttatt                                                                      <  1:91673/81‑4 (MQ=255)    cTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACtttat                                                                       <  1:91674/80‑3 (MQ=255)    cTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACtttat                                                                       <  1:91672/80‑3 (MQ=255)         ggATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTAt                                                                 <  2:91675/80‑1 (MQ=255)          gATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTAttt                                                               >  2:91697/1‑81 (MQ=255)                aCAGCGCAGAATTACAGCCACTGAGCAGTACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTcc                                                             <  2:91676/77‑1 (MQ=255)                aCAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCt                                                            <  2:91677/78‑1 (MQ=255)                 cAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAGTAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGt                                                        <  2:91679/81‑1 (MQ=255)                 cAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGt                                                        <  1:91678/81‑1 (MQ=255)                   gcgcAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGtct                                                      <  1:91680/81‑1 (MQ=255)                       aGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCAtt                                                  >  2:91695/1‑81 (MQ=255)                             aCAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACg                                             <  1:91681/80‑1 (MQ=255)                              cAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGAc                                           <  1:91682/81‑1 (MQ=255)                                   aCTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCaa                                      >  1:91696/1‑81 (MQ=255)                                       aGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTcc                                  <  2:91683/81‑1 (MQ=255)                                         cAATACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCAc                                <  1:91684/81‑1 (MQ=255)                                            tACAGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACggg                             <  2:91685/81‑1 (MQ=255)                                               aGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATc                          >  2:91702/1‑81 (MQ=255)                                               aGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATc                          >  1:91698/1‑81 (MQ=255)                                               aGTGCCTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTCAACGACCTCAATTCCACGGGATc                          >  1:91699/1‑81 (MQ=255)                                                   ccTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtgg                       <  1:91686/80‑1 (MQ=255)                                                    cTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtggtg                     <  1:91687/81‑1 (MQ=255)                                                    cTGCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCtggtg                     >  2:91706/1‑81 (MQ=255)                                                      gCAAATAATGACAACCATCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGc                   >  1:91700/1‑81 (MQ=255)                                                                       tCCCAAAC‑TTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGc  <  1:91688/81‑1 (MQ=255)                                                                               |                                                                          GTCCTTTGGGATCTAACAGCGCAGAATTACAGCCACTGAGCAATACAGTGCCTGCAAATAATGACAACCATCCCAAACTTTTATTGTATTTCCTGAGTCTCATTTAACGACCTCAATTCCACGGGATCTGGTGGCGTTTAAAGTGTGTGGGC  >  NC_000913/451508‑451659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑