Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	583,957	(T)6→5	coding (277/750 nt)	appY →	DNA‑binding transcriptional activator AppY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	583,952	0	T	.	100.0%	15.5 / NA	5	coding (272/750 nt)	appY	DNA‑binding transcriptional activator AppY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (3/2);  total (3/2)

ATGAGCTGCCTGGCAAATTTACTCTTGATAAGAATGGCTATATTGCTGAAGAGGAACCTCCATTATCGCTTGTTTTTTCTCTGTTTGAAGGGATTAAAATAGCAGACTCACACTCCCTTTGGTTAAAAGAAAGACTATGTATAT  >  NC_000913/583880‑584023                                                                         |                                                                        atGAGCTGCCTGGCAAATTTACTCTTGATAAGAATGGCTATATTGCTGAAGAGGAACCTCCATTATCGCTTGtttttctct                                                                 <  2:115454/81‑5 (MQ=255) atGAGCTGCCTGGCAAATTTACTCTTGATAAGAATGGCTATATTGCTGAAGAGGAACCTCCATTATCGCTTGtttttctct                                                                 >  2:605803/1‑77 (MQ=255)             gCAAATTTACTCTTGATAAGAATGGCTATATTGCTGAAGAGGAACCTCCATTATCGCTTG‑TTTTTCTCTGTTTGAAGGGa                                                     <  2:115455/80‑1 (MQ=255)                           gATAAGAATGGCTATATTGCTGAAGAGGAACCTCCATTATCGCTTG‑TTTTTCTCTGTTTGAAGGGATTAAAATAGCAGAcc                                      >  1:115471/1‑80 (MQ=255)                           gATAAGAATGGCTATATTGCTGAAGAGGAACCTCCATTATCGCTTG‑TTTTTCTCTGTTTGAAGGGATTAAAATAGCAGAc                                       <  2:115456/80‑1 (MQ=255)                                    ggCTATATTGCTGAAGAGGAACCTCCATTATCGCTTG‑TTTTTCTCTGTTTGAAGGGATTAAAATAGCAGACTCACACTccc                             >  2:115475/1‑81 (MQ=255)                                                               ttATCGCTTG‑TTTTTCTCTGTTTGAAGGGATTAAAATAGCAGACTCACACTCCCTTTGGTTAAAAGAAAGACTATGTatat  >  1:115468/1‑81 (MQ=255)                                                                         |                                                                        ATGAGCTGCCTGGCAAATTTACTCTTGATAAGAATGGCTATATTGCTGAAGAGGAACCTCCATTATCGCTTGTTTTTTCTCTGTTTGAAGGGATTAAAATAGCAGACTCACACTCCCTTTGGTTAAAAGAAAGACTATGTATAT  >  NC_000913/583880‑584023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑