Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,144	(T)8→7	intergenic (‑11/+369)	metT ← / ← asnB	tRNA‑Met/asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,137	0	T	.	100.0%	76.7 / NA	19	intergenic (‑4/+376)	metT/asnB	tRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/15);  total (4/15)

TACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTC  >  NC_000913/697064‑697206                                                                          |                                                                      tACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCtttttttc                                                                >  1:135636/1‑80 (MQ=255)   cgacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCtttttttc                                                                <  2:135585/79‑2 (MQ=255)   cgacgGGATTCCAACCTGTGACCCAATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCtttttttcgc                                                              >  2:135627/1‑78 (MQ=14)    gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCtttttttcgc                                                              >  2:135644/1‑77 (MQ=255)    gacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCtttttttcgc                                                              >  2:135637/1‑77 (MQ=255)              gAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATc                                                  <  1:135586/81‑1 (MQ=255)               aaCCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCg                                                 <  1:135587/81‑1 (MQ=255)                        ccccATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGGTACCTTATCGGCGTTGCgg                                        <  2:135588/81‑1 (MQ=255)                           catcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGgc                                     >  1:135638/1‑81 (MQ=255)                            atcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGgcg                                    <  2:135589/81‑1 (MQ=255)                             tcatTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGgcgc                                   <  2:135590/81‑1 (MQ=255)                                ttATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCAtt                                <  2:135591/81‑1 (MQ=255)                                  aTGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTAt                              >  1:135639/1‑81 (MQ=255)                                    gAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGc                            >  2:135641/1‑81 (MQ=255)                                      gTGATGTGCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGt                          <  2:135592/81‑1 (MQ=255)                                          tgtgCTCTAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATag                      <  2:135593/81‑1 (MQ=255)                                                ctAACCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCtt                >  1:135642/1‑81 (MQ=255)                                                   aCCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGc              <  2:135597/80‑1 (MQ=255)                                                   aCCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGc              <  2:135594/80‑1 (MQ=255)                                                   aCCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGc              <  1:135596/80‑1 (MQ=255)                                                   aCCAACTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCa             <  2:135595/81‑1 (MQ=255)                                                        cTGAGCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTc        <  1:135598/81‑1 (MQ=255)                                                            gCTACGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAAcc    <  2:135599/81‑1 (MQ=255)                                                                cGTAGCCATC‑TTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACctc  <  1:135600/79‑1 (MQ=255)                                                                          |                                                                      TACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCATCTTTTTTTTCGCGATACCTTATCGGCGTTGCGGGGCGCATTATGCGTATAGAGCCTTGCAGCGTCAACCTC  >  NC_000913/697064‑697206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑