| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 310 | (T)9→10 | intergenic (+55/‑27) | thrL → / → thrA | thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 301 | 1 | . | T | 93.8% | 109.4 / ‑0.6 | 32 | intergenic (+46/‑36) | thrL/thrA | thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (22/8); total (23/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGC > NC_000913/225‑371 | caccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑tttt > 1:46/1‑81 (MQ=255) accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑tttt < 1:13/80‑1 (MQ=255) aGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGAcc > 2:47/1‑80 (MQ=255) aGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGAcc > 1:50/1‑80 (MQ=255) ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCaa < 2:14/81‑1 (MQ=255) ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCa > 1:62/1‑80 (MQ=255) aaCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAgg > 1:60/1‑81 (MQ=255) aCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGt < 2:15/81‑1 (MQ=255) ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTaa > 2:67/1‑81 (MQ=255) ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTaa < 2:16/81‑1 (MQ=255) ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTaa > 1:66/1‑81 (MQ=255) gcgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTaacaac > 2:51/1‑80 (MQ=255) cgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCa < 1:17/81‑1 (MQ=255) cgTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCa > 1:59/1‑81 (MQ=255) cAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGc > 1:92/1‑80 (MQ=255) ggAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg > 1:73/1‑81 (MQ=255) gAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt > 1:68/1‑81 (MQ=255) aaaCACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgtg > 1:63/1‑81 (MQ=255) caGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg > 2:56/1‑79 (MQ=255) aaaaaaGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGtt < 1:19/81‑1 (MQ=255) aaaaaGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc > 1:61/1‑81 (MQ=255) aaaGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg > 1:57/1‑81 (MQ=255) aGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcg > 2:64/1‑81 (MQ=255) aGCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcg > 2:58/1‑81 (MQ=255) gCCCGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcgg > 2:83/1‑81 (MQ=255) cccGCACCTGACAGTGCGGGC‑TTTTGTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGt < 2:20/81‑1 (MQ=255) ccGCACCTGACAGTGCGGGC‑GTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa > 1:55/1‑80 (MQ=255) cGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa > 1:76/1‑80 (MQ=255) cGCACCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAc < 1:21/81‑1 (MQ=255) aCCTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt > 1:70/1‑80 (MQ=255) cTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAGGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAg < 2:22/81‑1 (MQ=255) cTGACAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAg > 1:80/1‑81 (MQ=255) aCAGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTg < 1:23/80‑1 (MQ=255) aGTGCGGGCTTTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc > 1:82/1‑80 (MQ=255) | CACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTT‑TTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGC > NC_000913/225‑371 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |