Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	844,966	(T)7→8	coding (515/2226 nt)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	844,959	1	.	T	100.0%	50.5 / NA	15	N174K (AAT→AAA)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (7/8);  total (7/8)

AGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGA‑TTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGC  >  NC_000913/844882‑845022                                                                               |                                                                aGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGA‑tt                                                               <  1:160904/80‑1 (MQ=255)        gTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGA‑ttttttttgc                                                       <  2:160905/81‑4 (MQ=255)         tAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGA‑ttttttttgcc                                                      >  2:160929/1‑77 (MQ=255)                 ggTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCAc                                               <  2:160906/81‑1 (MQ=255)                 ggTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCAc                                               <  2:160907/81‑1 (MQ=255)                  gTCGATAATCAACGCCCCGATTAACGTCACCTGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACt                                              <  1:898144/81‑1 (MQ=255)                    cGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGa                                            >  2:160932/1‑81 (MQ=255)                            aaCGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCAtttt                                    >  1:160937/1‑81 (MQ=255)                             aCGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTg                                   <  2:160908/81‑1 (MQ=255)                                     aTAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAg                           >  2:160934/1‑81 (MQ=255)                                      tAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGc                          <  2:160909/81‑1 (MQ=255)                                              gCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTgc                  >  1:160936/1‑81 (MQ=255)                                                  gAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGcac              <  1:160910/81‑1 (MQ=255)                                                  gAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGcac              <  1:160911/81‑1 (MQ=255)                                                  gAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGca               >  2:160933/1‑80 (MQ=255)                                                    aGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAg            <  1:160912/81‑1 (MQ=255)                                                           aCCAGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAAt      >  1:160931/1‑80 (MQ=255)                                                              aGTTGCTGCGCTCACGATTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGc  >  2:160935/1‑81 (MQ=255)                                                                               |                                                                AGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGA‑TTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGC  >  NC_000913/844882‑845022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑