Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,231	(A)8→9	intergenic (‑276/+11)	bssS ← / ← dinI	regulator of biofilm formation/DNA damage‑inducible protein I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,223	1	.	A	100.0%	39.7 / NA	12	intergenic (‑268/+19)	bssS/dinI	regulator of biofilm formation/DNA damage‑inducible protein I
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (10/2);  total (10/2)

GAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTG  >  NC_000913/1121159‑1121286                                                                  |                                                                gAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTg                                                   <  2:203943/80‑1 (MQ=255)   ggAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCa                                                 >  1:203967/1‑80 (MQ=255)            gAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGc                                       >  1:203953/1‑81 (MQ=255)            gAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGc                                       >  2:203960/1‑81 (MQ=255)             aaaTTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCt                                      <  1:203944/81‑1 (MQ=255)               aTTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGa                                    >  2:203962/1‑81 (MQ=255)                   tGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACaaa                                >  2:203971/1‑81 (MQ=255)                    gTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAAc                               >  1:203965/1‑81 (MQ=255)                          aaaaTTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCa                         >  1:203957/1‑81 (MQ=255)                           aaaTTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCAt                        >  2:203966/1‑81 (MQ=255)                                                 aGCCGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTg  >  2:203959/1‑81 (MQ=255)                                                   ccGGGGCGACCCGGCAAAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTg  >  1:203952/1‑79 (MQ=255)                                                                  |                                                                GAGGAAGTGTAGAAATTTTGTACTCAAAATTCGTAAGTAAAATAAAAAAGCCGGGGCGACCCGGC‑AAAAAAAATCACTGCATATTATTCGCTGACAAACCAGTCATCGGCGCTTTCCCACGTTTCCTG  >  NC_000913/1121159‑1121286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑