| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,133,116 | (A)6→7 | coding (543/1209 nt) | flgE → | flagellar hook protein FlgE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,133,110 | 1 | . | A | 94.1% | 54.8 / ‑2.9 | 17 | coding (537/1209 nt) | flgE | flagellar hook protein FlgE |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (8/8); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGCAGATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAAC‑AAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTG > NC_000913/1133032‑1133176 | gATGCAGATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAAC‑a > 2:205749/1‑80 (MQ=255) aaTTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTg < 2:205714/79‑1 (MQ=255) aTTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACt > 2:205733/1‑81 (MQ=255) ttCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTg < 1:205715/81‑1 (MQ=255) cAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGttt < 1:205716/81‑1 (MQ=255) aTCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGAc < 2:205717/81‑1 (MQ=255) cGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGt < 2:205719/81‑1 (MQ=255) cGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGt < 2:205718/81‑1 (MQ=255) gCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGt > 2:205735/1‑80 (MQ=255) gCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTc < 1:205720/81‑1 (MQ=255) ttCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAg > 1:205745/1‑81 (MQ=255) tGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAA‑AGGCTCGGTGACTGTTTTCGACAGTCAGGGTAATg > 2:205751/1‑81 (MQ=255) ccATTCAGCGCCAGCAATGCGGATAGCTATAAC‑AAAAAGAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTcatga > 1:205742/1‑81 (MQ=255) gcgcCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGc > 1:205741/1‑81 (MQ=255) cgcCAGCAATGCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCg < 1:205721/81‑1 (MQ=255) gCGGATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTtgt > 1:205744/1‑81 (MQ=255) gATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTtgtg > 1:205752/1‑79 (MQ=255) gATAGCTATAACAAAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTtgtg > 1:205736/1‑79 (MQ=255) | GATGCAGATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAAC‑AAAAA‑AGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTG > NC_000913/1133032‑1133176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |