| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,157,840 | (A)7→8 | intergenic (+266/‑29) | ycfH → / → ptsG | putative metal‑dependent hydrolase YcfH/glucose‑specific PTS enzyme IIBC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,157,833 | 1 | . | A | 92.3% | 38.7 / ‑2.8 | 13 | intergenic (+259/‑36) | ycfH/ptsG | putative metal‑dependent hydrolase YcfH/glucose‑specific PTS enzyme IIBC component |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (7/5); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGT‑AAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGT > NC_000913/1157762‑1157907 | tgtAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGT‑aaaaaaaag > 2:209611/1‑79 (MQ=255) gtAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGT‑aaaaaaaag > 2:209613/1‑78 (MQ=255) ataaataaAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCAcc < 2:209583/81‑1 (MQ=255) ataaataaAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCAcc > 2:209607/1‑81 (MQ=255) aaataaaGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCa > 1:209612/1‑81 (MQ=255) cgcTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAg > 1:209606/1‑81 (MQ=255) cccTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAAt < 1:209584/81‑1 (MQ=255) gTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATg > 1:209614/1‑81 (MQ=255) gAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAAt > 1:209609/1‑81 (MQ=255) ggCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGc < 1:209585/81‑1 (MQ=255) tGCCACGCGTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTg > 1:209616/1‑81 (MQ=255) gCCACGCGTGAGAACGA‑AAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTg < 1:209586/79‑1 (MQ=255) cgTGAGAACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAgg < 1:209587/81‑1 (MQ=255) gaACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCgg > 2:209615/1‑80 (MQ=255) gaACGTAAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGt < 2:209588/81‑1 (MQ=255) | TGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGT‑AAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGT > NC_000913/1157762‑1157907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |