Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,229,560	(T)7→8	coding (835/912 nt)	hlyE ←	hemolysin E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,229,553	1	.	T	100.0%	26.8 / NA	9	M281K (ATG→AAG)	hlyE	hemolysin E
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (5/4);  total (5/4)

AAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGT  >  NC_000913/1229501‑1229626                                                      |                                                                          aaaGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCaaag                                                <  2:219100/81‑1 (MQ=255) aaaGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCaaag                                                <  2:219101/81‑1 (MQ=255)      gtgtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCaaagaaag                                            >  2:219116/1‑80 (MQ=255)         tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAtt                                        <  1:219102/81‑1 (MQ=255)           tttttACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTa                                       >  1:219113/1‑80 (MQ=255)                       cTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                           >  1:219114/1‑80 (MQ=255)                       cTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                           >  2:219117/1‑80 (MQ=255)                                  cTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATc               <  1:219103/81‑1 (MQ=255)                                               ttAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGt  >  1:219120/1‑81 (MQ=255)                                                      |                                                                          AAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGT  >  NC_000913/1229501‑1229626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑