Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,341,740	(A)8→9	intergenic (+13/‑181)	lapB → / → pyrF	lipopolysaccharide assembly protein B/orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,341,732	1	.	A	100.0%	42.8 / NA	13	intergenic (+5/‑189)	lapB/pyrF	lipopolysaccharide assembly protein B/orotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (5/8);  total (5/8)

TTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAA  >  NC_000913/1341676‑1341807                                                          |                                                                            ttGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTa                                                       >  1:234424/1‑80 (MQ=255)   gTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTa                                                       <  1:234397/78‑1 (MQ=255)        gCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATAc                                               <  1:234398/81‑1 (MQ=255)           tGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTaa                                            >  2:234421/1‑81 (MQ=255)            ggTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAat                                           >  1:234418/1‑81 (MQ=255)                    aTTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATg                                   >  2:234422/1‑81 (MQ=255)                        aaCCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTcc                               <  2:234400/81‑1 (MQ=255)                        aaCCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTc                                <  1:234399/80‑1 (MQ=255)                              ttCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATtgtg                         <  2:234401/81‑1 (MQ=255)                                         gATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCa                 <  2:234403/78‑1 (MQ=255)                                         gATGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAac               <  2:234402/80‑1 (MQ=255)                                           tGGCCTGTAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAacgac            >  1:234430/1‑81 (MQ=255)                                                  tAATTTTTAAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAg     <  2:234404/81‑1 (MQ=255)                                                    ttttta‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGaaa  <  1:234405/80‑1 (MQ=255)                                                      ttta‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGaaa  >  2:234433/1‑79 (MQ=255)                                                          |                                                                            TTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTT‑AAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGACGGCGCAGAAA  >  NC_000913/1341676‑1341807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑