Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,676,370	+C	intergenic (‑10/+1)	pntB ← / ← pntA	pyridine nucleotide transhydrogenase subunit beta/pyridine nucleotide transhydrogenase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,676,366	1	.	C	100.0%	33.0 / NA	11	intergenic (‑6/+5)	pntB/pntA	pyridine nucleotide transhydrogenase subunit beta/pyridine nucleotide transhydrogenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (7/4);  total (7/4)

CGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTA‑CCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGTCACGGTGAAGCCACCGAAAATA  >  NC_000913/1676286‑1676428                                                                                  |                                                               cGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTa                                                                 >  2:274501/1‑81 (MQ=255)  gAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTa                                                                 <  2:274480/80‑1 (MQ=255)     aGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTA‑cccc                                                            <  1:274481/81‑1 (MQ=255)         cGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTA‑ccccctta                                                        <  2:274482/81‑5 (MQ=255)             cAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAAttt                                                     >  2:274494/1‑81 (MQ=255)                      gATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAAc                                            <  2:274483/81‑1 (MQ=255)                              ggATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAg                                    <  1:274484/81‑1 (MQ=255)                                aTCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAGCa                                  >  2:274498/1‑81 (MQ=255)                                   gcgGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAGCATgc                               <  1:274485/81‑1 (MQ=255)                                     ggCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAGCATgcgc                             >  2:274504/1‑81 (MQ=255)                                          caaTGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGt                        >  1:274506/1‑81 (MQ=255)                                           aaTGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGTc                       <  1:274486/81‑1 (MQ=255)                                                aTGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGTCACGGt                  >  1:274505/1‑81 (MQ=255)                                                             tAATCCTCCAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGTCACGGTGAAGCCACCGaa      >  1:274510/1‑80 (MQ=255)                                                                tcctccAGACATATGTTACCCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGTCACGGTGAAGCCACCGAAAata  >  1:274502/1‑81 (MQ=255)                                                                                  |                                                               CGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTA‑CCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGTCACGGTGAAGCCACCGAAAATA  >  NC_000913/1676286‑1676428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑